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Series GSE65083 Query DataSets for GSE65083
Status Public on Nov 14, 2016
Title Bach2 inhibits Th2-type immune responses by regulating glutamine metabolism [expression array]
Organism Mus musculus
Experiment type Expression profiling by array
Summary It is becoming clear that the acquisition of T cell functions is closely linked to the reprogramming of the metabolic pathway. However, the impact of metabolic changes on the differentiation of helper T cell subsets remains unclear. We herein demonstrate a critical role of glutamine metabolism in regulating type 2-immune response. Bach2, a transcriptional repressor, binds to an AP-1 motif, and suppresses Th2 cell differentiation and glutaminolysis. Glutaminase 2, which controls glutamine metabolism, was identified as a Bach2 target gene. The pharmacological inhibition of glutamine metabolism normalized allergic lung inflammation that developed spontaneously in T-cell specific Bach2-deficient mice. These findings reveal the importance of the Bach2-dependent modulation of glutamine metabolism in regulating Th2 cell differentiation and Th2 cell- mediated inflammation.
 
Overall design Gene expression in Bach2 WT-Th (Bach2 WT-Th (-)), Bach2 KO-Th (Bach2 KO-Th (-)), restimulated Bach2 WT-Th (Bach2 WT Th (+)) and re-stimulated Bach2 KO-Th (Bach2 KO Th (+)).
 
Contributor(s) Kuwahara M, Izumoto M, Suzuki J, Ochi M, Shioiri K, Matsumoto A, Yamada T, Shinoda K, Nakayama T, Kurosaki T, Sawazaki T, Ohara O, Yasukawa M, Yamashita M
Citation(s) 27581382
Submission date Jan 19, 2015
Last update date Jan 12, 2017
Contact name Makoto Kuwahara
E-mail(s) mkuwa@m.ehime-u.ac.jp
Organization name Graduate School of Medicine, Ehime University
Department Department of Immunology
Lab Immunology
Street address Shitsukawa
City Toon-shi
State/province Ehime
ZIP/Postal code 791-0295
Country Japan
 
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (4)
GSM1586921 Bach2_WT_IL-2_(-)
GSM1586922 Bach2_KO_IL-2_(-)
GSM1586923 Bach2_WT_IL-2_(+)
This SubSeries is part of SuperSeries:
GSE65084 Bach2-Batf interactions control Th2-type immune responses by regulating the IL-4 amplification loop
Relations
BioProject PRJNA272912

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65083_RAW.tar 8.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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