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| Status |
Public on Sep 25, 2015 |
| Title |
Effect of cortistatin A (CA) on enhancer occupancy in CA-sensitive and -insensitive human cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We characterized the marine natural product cortistatin A (CA) as an inhibitor of CDK8 to determine whether pharmacologic inhibition of CDK8 regulates super-enhancer function and inhibits AML proliferation.
In this series, we use ChIP-seq of H3K27ac, H3K4me1, MED1, CDK8, BRD4, and RNA polymerase II (PolII) to examine enhancer occupancy and transcriptional response to CA in human cells lines.
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| Overall design |
ChIP-seq was carried out in CA-sensitive: MOLM-14 cells (H3K27Ac, H3K4me1, MED1, CDK8, BRD4, and PolII), MV4;11(H3K27Ac, CDK8), SET2 (H3K27Ac, CDK8), and in CA-insensitive cells: K562 (CDK8).
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| Contributor(s) |
Pelish HE, Liau BB, Nitulescu II, Lemieux ME, Shair MD |
| Citation(s) |
26416749 |
| Submission date |
Jan 21, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Matthew D. Shair |
| Organization name |
Harvard University
|
| Department |
Chemistry and Chemical Biology
|
| Lab |
Shair
|
| Street address |
12 Oxford Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
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| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (53)
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| This SubSeries is part of SuperSeries: |
| GSE65161 |
Mediator kinase inhibition further activates super-enhancer-associated genes in AML |
|
| Relations |
| BioProject |
PRJNA273301 |
| SRA |
SRP052715 |
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