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Series GSE6514 Query DataSets for GSE6514
Status Public on Feb 11, 2007
Title Gene expression in the mouse brain during spontaneous sleep and prolonged wakefulness
Organism Mus musculus
Experiment type Expression profiling by array
Summary These studies address temporal changes in gene expression during spontaneous sleep and extended wakefulness in the mouse cerebral cortex, a neuronal target for processes that control sleep; and the hypothalamus, an important site of sleep regulatory processes. We determined these changes by comparing expression in sleeping animals sacrificed at different times during the lights on period, to that in animals sleep deprived and sacrificed at the same diurnal time.
Keywords: gene expression, temporal changes, brain, behavior, sleep,
Overall design Experiments were performed on male mice (C57/BL6), 10 weeks of age ±1 week. Animals were housed in a light/dark cycle of 12 hrs, in a pathogen free, temperature- and humidity-controlled room (22°C and 45-55%, respectively) with water available ad libitum. Food was accessible for 12 hrs only during the active period. Animals were subjected to 14 days of acclimatization during which a nighttime feeding pattern was established. This was done to avoid differential food intake between mice that were subsequently sleep deprived during the lights on period and those allowed to sleep. Mice were sacrificed following 3, 6, 9 and 12 hrs of total sleep deprivation (n=5 at each time point). Deprivation was initiated at lights-on, and performed through gentle handling. Sleeping animals, which were left undisturbed, were sacrificed at the same diurnal time points as sleep deprived mice (n=5 at each time point). An additional control group of mice were sacrificed at time zero, i.e., at the time of lights-on (n=5). All mice were behaviorally monitored using the AccuScan infrared monitoring system that detects movement when the mouse crosses electronic beams (Columbus Instruments). Mice were sacrificed by cervical dislocation. Brain sectioning was performed according to the mouse brain atlas of Franklin and Paxinos . The primary and secondary motor areas (M1 and M2) of the cerebral cortex and broadly defined regions and zones of the hypothalamus were sampled. RNA was isolated with Trizol (Invitrogen) and further purified using RNeasy columns (Qiagen) as per the manufacturer's instructions.
Contributor(s) Mackiewicz M, Shockley KR, Romer MA, Galante RJ, Zimmerman JE, Naidoo N, Baldwin DA, Jensen ST, Churchill GA, Pack AI
Citation(s) 17698924
Submission date Dec 12, 2006
Last update date Feb 11, 2019
Contact name Miroslaw Mackiewicz
Phone (215) 746-4805
Fax (215) 746-4814
Organization name University of Pennsylvania
Department Medicine
Lab Division of Sleep Medicine
Street address 2124 TRL 125S 31st Street
City Philadelphia
State/province PA
ZIP/Postal code 19104-3403
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (90)
GSM149511 1_cerebral_cortex_time_0_#37
GSM149512 2_cerebral_cortex_time_0_#39
GSM149513 3_cerebral_cortex_time_0_#40
BioProject PRJNA98599

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Supplementary file Size Download File type/resource
GSE6514_RAW.tar 348.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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