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Series GSE6526 Query DataSets for GSE6526
Status Public on Mar 01, 2007
Title Expression time course data HEY2 KO and WT MASMC treated with PDGF
Organism Mus musculus
Experiment type Expression profiling by array
Summary The cardiovascular restricted transcription factor CHF1/Hey2 has been previously shown to regulate the smooth muscle response to growth factors. To determine how CHF1/Hey2 affects the smooth muscle response to growth factors, we performed a genomic screen for transcripts that are differentially expressed in wild type and knockout smooth muscle cells after stimulation with platelet derived growth factor.
We screened 45101 probes representing more than 39,000 transcripts derived from at least 34,000 genes, at eight different time points. We analyzed the expression data utilizing an algorithm based on Bayesian statistics to derive the best polynomial clustering model to fit the expression data. We found that in a total of 9827 transcripts the normalized ratio of knockout to wild type expression diverged more than 3 fold from baseline in at least one time point, and these transcripts separated into 17 distinct clusters. Further analysis of each cluster revealed distinct alterations in gene expression patterns for immediate early genes, transcription factors, matrix metalloproteinases, signaling molecules and other molecules important in vascular biology.
Keywords: time course
 
Overall design WT and Hey2 knockout mouse aortic smooth muscle cells were treated in parallel with PDGF and harvested at successive time points for RNA extraction and hybridization on Affymetrix microarrays. We sought to identify differentially expressed transcripts through Bayesian statistical methods that would explain the differential response to PDGF observed in vitro.
 
Contributor(s) Shirvani SM, Mookanamparambil L, Ramoni MF, Chin MT
Citation(s) 17327490
Submission date Dec 14, 2006
Last update date Feb 11, 2019
Contact name Michael T Chin
E-mail(s) mtchin@u.washington.edu
Organization name University of Washington
Department Medicine
Street address 815 Mercer Street
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (16)
GSM150243 MASMC_Hey2_KO_PDGF_T0
GSM150244 MASMC_Hey2_KO_PDGF_T120
GSM150245 MASMC_Hey2_KO_PDGF_T15
Relations
BioProject PRJNA98801

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6526_RAW.tar 59.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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