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| Status |
Public on Mar 01, 2016 |
| Title |
Single Cell triple omics sequencing reveals genetic and epigenetic heterogeneity in hepatocellular carcinoma |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
|
| Summary |
Single cell genome, DNA methylome, and transcriptome sequencing has been achieved separately. However, to analyze the regulation of RNA expression by genetic and epigenetic factors within an individual cell, it is necessary to analyze these omics simultaneously from the same single cell. Here we developed a single cell triple omics sequencing technique- scTrio-seq, to analyze the genome, DNA methylome, and transcriptome concurrently of a mammalian cell.
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| Overall design |
6 single human HepG2 cell line cells were sequenced using the newly developed scTrio-seq, other 2 HepG2 cells were sequenced using scRNA-seq and other 2 HepG2 cells were sequenced using scRRBS as technique control. 6 single mouse embryonic stem cells (mESCs) were sequenced using the newly developted scTrio-seq. Meanwhile, two scRNA-seq and two scRRBS were also completed using two mESCs separately. 26 single cells from hepatocellular carcinoma were sequenced using scTrio-seq to analyze the regulation relations between three omics of cancer cells.
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| |
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| Contributor(s) |
Hou Y, Tang F, Huang Y, Peng J |
| Citation(s) |
26902283 |
| Submission date |
Jan 28, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Yu Hou |
| Organization name |
Peking University
|
| Department |
School of life sciences
|
| Lab |
Fuchou Tang
|
| Street address |
No.5 Yiheyuan Street
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
| |
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| Platforms (4)
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| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (95)
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| Relations |
| BioProject |
PRJNA273802 |
| SRA |
SRP052901 |
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