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Series GSE65517 Query DataSets for GSE65517
Status Public on Feb 03, 2015
Title Comparative analysis of monocytes from healthy donors, patients with metastatic breast cancer, sepsis or tuberculosis.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Myeloid-derived suppressor cells (MDSCs) are highly immunosuppressive myeloid cells, which increase in cancer patients. The molecular mechanism behind their generation and function is unclear. Whereas granulocytic-MDSCs correlate with poor overall survival in breast cancer, the presence and relevance of monocytic-MDSCs (Mo-MDSCs) is unknown. Here we report for the first time an enrichment of functional blood Mo-MDSCs in breast cancer patients before they acquire a typical Mo-MDSC surface phenotype. A clear population of Mo-MDSCs with the typical cell surface phenotype (CD14+HLA-DRlow/-Co-receptorlow/-) increased significantly first during disease progression and correlated to metastasis to lymph nodes and visceral organs. Furthermore, monocytes, comprising the Mo-MDSC population, from patients with metastatic breast cancer resemble the reprogrammed immunosuppressive monocytes in patients with severe infections, both by their surface and functional phenotype but also at their molecular gene expression profile. Our data suggest that monitoring the Mo-MDSC levels in breast cancer patients may represent a novel and simple biomarker for assessing disease progression.
 
Overall design Peripheral blood monocytes were isolated using magnetic cell sorting from 4 patients with metastatic breast cancer, 3 healthy controls, 3 patients with sepsis and 3 patients with active tuberculosis were immediately frozen at -80┬░C in TRIZOL.
 
Contributor(s) Bergenfelz C, von Stedingk K, Gruvberger-Saal S, Leandersson K
Citation(s) 25992611
Submission date Feb 02, 2015
Last update date Aug 13, 2018
Contact name Kristoffer von Stedingk
E-mail(s) kristoffer.von_stedingk@med.lu.se
Organization name Lund University
Street address Scheelev├Ągen 2
City Lund
ZIP/Postal code 223 81
Country Sweden
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (13)
GSM1599177 Primary human monocytes from patient with metastatic breast cancer #1
GSM1599178 Primary human monocytes from patient with metastatic breast cancer #2
GSM1599179 Primary human monocytes from patient with metastatic breast cancer #3
Relations
BioProject PRJNA274264

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65517_RAW.tar 26.2 Mb (http)(custom) TAR
GSE65517_non-normalized.txt.gz 1.1 Mb (ftp)(http) TXT
GSE65517_normalized.txt.gz 1.6 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table
Processed data are available on Series record

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