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Series GSE65528 Query DataSets for GSE65528
Status Public on Sep 07, 2015
Title Single cell time course of macrophages exposed to Salmonella enterica subsp. typhimurium
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We present a detailed single cell time course of the macrophage response to Salmonella infection. By combining phenotypic fluorescent labels with single cell expression analysis we are able to identify gene modules associated with bacterial exposure and bacterial infection. We also identify other genetic clusters that are expressed heterogenously, ananlyzing both their regulation and their impact on infection
 
Overall design Analysis of 192 single cells across 4 time points after Salmonella exposure (MOI 1:1) with one of three different fluorescent labels indicating whether a given cell contained no intracellular bacteria (non-fluorescent), contained dead intracellular bacteria (only pHrodo positive), or contained live intracellular bacteria (pHrodo and GFP positive)
 
Contributor(s) Avraham R, Haseley N, Brown D, Penaranda C, Jijon HB, Trombetta JJ, Satija R, Shalek AK, Xavier R, Regev A, Hung DT
Citation(s) 26343579
Submission date Feb 02, 2015
Last update date May 15, 2019
Contact name Deborah T Hung
Organization name Broad Institute
Department Infectious Disease Initiative
Lab Hung lab
Street address 415 Main Street
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (192)
GSM1599514 macrophage (exposed to S. enterica) 1
GSM1599515 macrophage (exposed to S. enterica) 2
GSM1599516 macrophage (exposed to S. enterica) 3
Relations
BioProject PRJNA274277
SRA SRP053053

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65528_s121_p1_Counts_table.txt.gz 1.7 Mb (ftp)(http) TXT
GSE65528_s121_p1_TPM_table.txt.gz 2.2 Mb (ftp)(http) TXT
GSE65528_s121_p2_Counts_table.txt.gz 1.5 Mb (ftp)(http) TXT
GSE65528_s121_p2_TPM_table.txt.gz 1.9 Mb (ftp)(http) TXT
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