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| Status |
Public on Sep 07, 2015 |
| Title |
Analysis of bacterial and macrophage transcripts in ISRE-positive and negative infected macrophages |
| Organisms |
Mus musculus; Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To identify bacterial transcripts that may be associated with type I IFN production in Salmonella enterica subsp typhimurium (SL1344) infected macrophages we transformed macrophages with an ISRE-GFP reporter construct and sorted separate populations of GFP positive and GFP negative infected macrophages. We then did whole transcriptome profiling, collecting both host and bacterial transcripts, for differential expression analysis
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| Overall design |
Analysis of ISRE positive, negative, and mixed populations at two time points (unexposed and 24hours) in duplicate (biological replicates). A sample consisting of Salmonella prior to infection was also included
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| Contributor(s) |
Avraham R, Haseley N, Brown D, Penaranda C, Jijon HB, Trombetta JJ, Satija R, Shalek AK, Xavier R, Regev A, Hung DT |
| Citation(s) |
26343579 |
| Submission date |
Feb 02, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Deborah T Hung |
| Organization name |
Broad Institute
|
| Department |
Infectious Disease Initiative
|
| Lab |
Hung lab
|
| Street address |
415 Main Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
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| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19729 |
Illumina HiSeq 2500 (Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA274280 |
| SRA |
SRP053056 |
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