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| Status |
Public on Jan 02, 2007 |
| Title |
Expression data from primary human keratinocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Oncogenic Ras induces epidermal cell growth arrest. Induction of the JNK/Ap1 signaling cascade by expression of MKK7 overcomes Ras-induced cell growth arrest in a manner dependent on AP1 fucntion.
We used microarrays to detail the global programme of gene expression in response to MKK7, Ras activation and AP1 inhibition
Keywords: response to specific genetic change
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| |
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| Overall design |
To study the gene expression profile responsive to Ras and MKK7 expression. We used retroviral expression system to express LacZ, MKK7, Ras, DNc-Jun, MKK7+Ras, and MKK7+Ras +DNc-Jun in primary human keratinocytes, and then extract total RNA from the transduced cells for gene expression analysis.
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| Contributor(s) |
Zhang JY, Adams AA, Ricky TW, Tao S, Khavari PA |
| Citation(s) |
17440097 |
| Submission date |
Dec 18, 2006 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Jennifer Yunyan Zhang |
| E-mail(s) |
jennifer.zhang@duke.edu
|
| Phone |
919-684-6794
|
| Organization name |
Duke University
|
| Department |
Dermatology
|
| Lab |
Zhang
|
| Street address |
3 Genome Ct, rm4130
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (6)
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| GSM151731 |
Primary human keratinocytes transduced to express LacZ control |
| GSM151732 |
Primary human keratinocytes transduced to expressMKK7 |
| GSM151733 |
Primary human keratinocytes transduced to express Ras |
| GSM151734 |
Primary human keratinocytes transduced to express dominant negative c-Jun (DNc-Jun) |
| GSM151735 |
Primary human keratinocytes transduced to express MKK7 and Ras |
| GSM151736 |
Primary human keratinocytes transduced to express MKK7, Ras and DNc-Jun |
|
| Relations |
| BioProject |
PRJNA98775 |
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