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| Status |
Public on Aug 01, 2008 |
| Title |
Target genes associated to siRNA mediated repression of Pea3 and Erm transcription factors in mammary cancerous cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Pea3 and Erm are transcription factors belonging to the ETS family which are involved in tumorigenesis, particularly mammary oncogenesis. To elucidate the mechanism of Pea3/Erm induced tumorigenesis in mouse mammary cancerous MMT cells (ATCC CCL-51), we have down-regulated Pea3 or Erm expression in this cell line by using small interfering RNA and performed a microarray analysis using Applied Biosystems AB1700 technology to define target associated genes.
Keywords: Comparative transcriptome hybridization, small interfering RNA, mouse mammary cancerous cells
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| Overall design |
Mouse mammary cancerous MMT cells (ATCC CCL-51) have been transfected with a siRNA control, an erm-directed siRNA or an pea3-directed siRNA. MMT cells were also used to determine the modifications due to the transfection protocol.
These conditions correspond to four sample.
Two biological replicate were used for the MMT cells (control sample), three biological replicates were used for the siRNA control (control sample) and erm-directed siRNA (reference sample) MMT cells and four biological replicates were used for the pea3-directed siRNA (reference sample) MMT cells. Transcriptome profiles (28.218 validated mouse genes) were acquired using Applied Biosystems AB1700 technology, which make use of chemiluminescence-based detection chemistry.
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| Contributor(s) |
Firlej V, Ladam F, Brysbaert G, de Launoit Y, Benecke A, Chotteau-Lelievre A |
| Citation(s) |
18827017 |
| Submission date |
Dec 21, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Anne Chotteau |
| E-mail(s) |
anne.chotteau@ibl.fr
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| Phone |
0033320871128
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| Fax |
0033320871111
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| Organization name |
CNRS UMR 8161
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| Lab |
Institut de Biologie de Lille
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| Street address |
1 rue du professeur calmette
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| City |
Lille |
| ZIP/Postal code |
59021 |
| Country |
France |
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| Platforms (1) |
| GPL2995 |
ABI Mouse Genome Survey Microarray |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA98897 |
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