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| Status |
Public on Aug 31, 2007 |
| Title |
Comparative analysis of gene expression in C57BL/6J and DBA/2J aging lungs. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We hypothesize that gene expression in the aging lungs of these two strains of mice are divergent thus contributing to the disparity in the phenotypes. More specifically, (1) Aging DBA/2J mice compared to aging C57BL/6 mice are known to be accelerated in their lung physiology and morphometry; (2) C57BL/6J are known to have longer natural longevity than DBA/2J mice. In order to test these hypotheses at the gene expression level, we utilized microarray analysis to examine transcriptional differences between aging lungs of both strains of mice.
Keywords: comparative expression profiling
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| Overall design |
This study utilizes microarray analysis to test these hypotheses. Three sets of lungs were harvested from both strains at each time point (C57BL/6J: 2, 18, AND 26 mos; DBA/2J: 2 and 18 mos). RNA was isolated and used for global gene expression profiling (Affymetrix Mouse 430 2.0 array). Statistically significant gene expression was determined as a minimum 6 counts of 9 pairwise comparisons, minimum 1.5-fold change, and p < 0.05. Further, Absolute | FC - FC SEM | >= 1.5.
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| Contributor(s) |
Misra V, Lee H, Singh A, Huang K, Thimmulappa R, Mitzner W, Biswal S, Tankersley C |
| Citation(s) |
17726092 |
| Submission date |
Dec 21, 2006 |
| Last update date |
Feb 11, 2019 |
| Contact name |
vikas misra |
| E-mail(s) |
vmisra@jhsph.edu
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| Organization name |
Johns Hopkins University School of public health
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| Department |
Environmental health sciences
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| Lab |
Clarke G Tankersley
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| Street address |
615 N. Wolfe street, E6609
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA98903 |
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