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Series GSE65927 Query DataSets for GSE65927
Status Public on Apr 21, 2015
Title Early postnatal expression data from mouse skeletal muscle stem cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Satellite cells are the primary source of stem cells for skeletal muscle growth and regeneration. Since adult stem cell maintenance involves a fine balance between intrinsic and extrinsic mechanisms, we performed genome-wide chronological expression profiling to identify the transcriptomic changes involved during early postnatal growth till acquisition of satellite cell quiescence.
 
Overall design Muscle samples were isolated from the trunk of Pax3GFP/+ mice. After digestion, GFP cells were purified via FACS and process for RNA extraction and hybridization on Affymetrix microarrays (Affymetrix Mouse Genome 430 2.0 Arrays).
The different ages selected for sample isolation were P1-P12-P28 (P, postnatal days), covering proliferant and quiescent satellite cells. The three stages were done in triplicate for P28 and 4 times for P1-P12, so 11 samples included in the microarray.
 
Contributor(s) Alonso-Martin S, Rochat A, de Reyniès A, Relaix F
Citation(s) 25742797
Submission date Feb 13, 2015
Last update date Feb 11, 2019
Contact name Sonia Alonso-Martin
E-mail(s) alonsomartin.s@gmail.com
Phone +33684321566
Organization name Myology Research Center U974-INSERM - UPMC-Paris VI - Institut de Myologie
Department Development and stem cells
Lab Team 8 - Relaix
Street address 105 bd de l'Hôpital
City Paris
ZIP/Postal code 75013
Country France
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (11)
GSM1611267 Postnatal P1, biological rep1
GSM1611268 Postnatal P1, biological rep2
GSM1611269 Postnatal P1, biological rep3
Relations
BioProject PRJNA275424

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Supplementary file Size Download File type/resource
GSE65927_RAW.tar 39.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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