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| Status |
Public on Oct 29, 2015 |
| Title |
Study of transcriptional effects of monosodium urate crystal (MSU) on HaCaT keratinocyte cell line. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Aim of the study was to characterize at a molecular level (changes in transcriptomes) the effect of monosodium urate crystal (MSU) on HaCaT keratinocyte cell line.
This was adressed by using a culture model. The HaCaT cell line (human keratinocytes) was stimulated by MSU (1mg/mL) vs control for 12 hrs. By using genome-wide expression profiling, we identified deregulation of functionally relevant gene networks.
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| Overall design |
HaCaT were obtained from Cell Lines Service (Eppelheim, Germany) and grown in DMEM medium (PAN biotech, Aidenbach, Germany) supplemented with 10% FBS (Life Technology, Grand Island, NY, USA), L-glutamine and non-essential amino acid. Before the treatment HaCaT cells were cultured in serum-free medium for 12hrs. HaCaT were treated with MSU (1mg/ml) vs DMEM control for 12hrs then submitted to RNA extration and gene expression profiling. Triplicate experiments were performed: HaCaT control (n=3), MSU-treated (n=3).
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| Contributor(s) |
Coulouarn C, Ondet T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 13, 2015 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Cedric Coulouarn |
| E-mail(s) |
cedric.coulouarn@inserm.fr
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| Organization name |
INSERM
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| Department |
U1242
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| Lab |
COSS
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| Street address |
CLCC Eugène Marquis, Rue de la Bataille Flandres Dunkerque, Bat D, 1er étage
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| City |
Rennes |
| ZIP/Postal code |
35042 |
| Country |
France |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA275435 |
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