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| Status |
Public on Apr 30, 2015 |
| Title |
Lineage conversion induced by pluripotency factors involves transient passage through an iPSC stage |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Brief expression of pluripotency-associated factors such as Oct4, Klf4, Sox2 and c-Myc (OKSM), in combination with differentiation-inducing signals, has been reported to trigger transdifferentiation of fibroblasts into other cell types. Here we show that OKSM expression in mouse fibroblasts gives rise to both induced pluripotent stem cells (iPSCs) and induced neural stem cells (iNSCs) under conditions previously shown to induce only iNSCs. Fibroblast-derived iNSC colonies silenced retroviral transgenes and reactivated silenced X chromosomes, both hallmarks of pluripotent stem cells. Moreover, lineage tracing with an Oct4-CreER labeling system demonstrated that virtually all iNSC colonies originated from cells transiently expressing Oct4, whereas ablation of Oct4+ cells prevented iNSC formation. Lastly, an alternative transdifferentiation cocktail that lacks Oct4 and was reportedly unable to support induced pluripotency yielded iPSCs and iNSCs carrying the Oct4-CreER-derived lineage label. Together, these data suggest that iNSC generation from fibroblasts using OKSM and other pluripotency-related reprogramming factors requires passage through a transient iPSC state.
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| Overall design |
5 samples were anlyzed in total, 2 induced pluripotent stem cell (iPSC) lines , 1 brain-derived neural stem cell (NSC) line and 2 induced NSC lines (iNSCs)
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| |
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| Contributor(s) |
Bar-Nur O, Hochedlinger K |
| Citation(s) |
26098450 |
| Submission date |
Feb 16, 2015 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Ori Bar-Nur |
| E-mail(s) |
ori.bar-nur@mail.huji.ac.il
|
| Organization name |
Harvard University
|
| Department |
Center of Regenerative medicine
|
| Lab |
Hochedlinger
|
| Street address |
185 Cambridge st.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
|
| Samples (5)
|
| GSM1611651 |
iPSCs derived using the BKSM polycistronic cassette #2 |
| GSM1611652 |
iPSCs derived using the BKSM polycistronic cassette #7 |
| GSM1611653 |
NSCs derived from E13.5 mouse brain |
| GSM1611654 |
iNSCs derived using the OKSM polycistronic cassette |
| GSM1611655 |
iNSCs derived using the BKSM polycistronic cassette |
|
| Relations |
| BioProject |
PRJNA275545 |
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