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| Status |
Public on Feb 21, 2015 |
| Title |
Effects of thyroxine on the Craniosynostotic Phenotype |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Thyroid hormone is know to effect growth and development. However little is know about the hormone thyroxine's effect on craniofacial growth and suture development.
We used microarray as one tool to determine gene expression profile of wild type and craniosynostotic (Twist 1 +/-) suture cells after thyroxine exposure.
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| Overall design |
We isolated whole RNA from Wild type C57BL6 and Twist 1 +/- suture derived calvarial cells and treated with proliferation media and with thyroxine 10^-6 mol/liter for 3 or 7 days in culture. We then used an Affymetrix array and compared expression profiles between control and experimental treatments.
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| Contributor(s) |
Cray JJ Jr, Barth JL |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 20, 2015 |
| Last update date |
Feb 21, 2018 |
| Contact name |
James Cray |
| E-mail(s) |
crayj@musc.edu
|
| Organization name |
Medical University of South Carolina
|
| Department |
Oral Health Sciences
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| Street address |
173 Ashley Avenue, BSB230B
|
| City |
Charleston |
| State/province |
South Carolina |
| ZIP/Postal code |
29425 |
| Country |
USA |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA275969 |
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