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| Status |
Public on Dec 23, 2015 |
| Title |
Intrinsic age-dependent changes and cell-cell contacts regulate nephron progenitor lifespan |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: To use single-cell RNA-Seq analysis of nephron progenitors in order to determine transcrptional differences as nephron progenitors age.
Methods: Using a combination of FACS sorting and a Fluidigm Single-cell auto-prep system, we generated high-throughput RNA-SEQ data of nephron progenitors during development
Results: Single cells transcriptome profiling of nephron progenitors revealed progressive age-dependent changes with heterogeneity increasing in older populations.
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| Overall design |
96-single cell transcriptomes were determined from nephron progenitors of e14.5, e18.5 and P0 using Cited1GFP transgenic animals
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| |
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| Contributor(s) |
Chen S, Kopan R, Brunskill EW |
| Citation(s) |
26460946 |
| Submission date |
Feb 23, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Raphael Kopan |
| E-mail(s) |
raphael.kopan@cchmc.org
|
| Organization name |
Cincinnati Children's Hospital
|
| Department |
Developmental Biology
|
| Lab |
Kopan Lab
|
| Street address |
3333 Burnet Avenue
|
| City |
Cincinnati |
| State/province |
Ohio |
| ZIP/Postal code |
45011 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (91)
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| Relations |
| BioProject |
PRJNA276103 |
| SRA |
SRP055425 |
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