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| Status |
Public on Jun 01, 2008 |
| Title |
Gene expression profiles in human malignant melanoma cell lines exposed to carbon ions or X-rays |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Little is known about molecular mechanism for its effectiveness of C-ions in melanomas. In this study, we examined the cytotoxic effects of C-ions on 6 human malignant melanoma cell lines, and gene expression profiles and cell cycle progression were also examined to reveal the mechanism of the therapeutic effectiveness of C-ions.
Equivalent doses of the C-ions were more effective at reducing the survival rates than X-rays, and the relative biological effectiveness (RBE) was > 2.0 in all cell lines.
In unsupervised 2D clustering performed for C-ions irradiated groups at 1 h after irradiation, many of the probes were down-regulated among 6 cell lines.
We identified probes, such as XKRY, ELA3A, T-STAR, SCN9A, ELA2A, CRK7, CCNA2, CROP, CYR61, SDFR1, and SMAD4 differentially responded to C-ions and X-rays among 4 cell lines. CCNA2 was down-regulated by C-ions more than X-rays at 3 h after irradiation, and C-ions induced G2/M arrest more than X-rays in three cell lines at 30 h.
Many of p53 target genes, such as ATF3, BTG2, CDKN1A, GADD45A, SESN1, and TNFRSF6 were up-regulated by C-ions at 3 h.
The expressions of p53 target genes were not changed in HMV-I and MeWo. In MeWo, APG3L, RPL26 and PCNA were down-regulated or E2F2 and LIMS3 were up-regulated, and in HMV-I, ATF3, FOS, and CYR61 were up-regulated at 1 h after C-ions.
In conclusion, C-ions affected p53-dependent and p53-independent pathways of cell death and cell cycle. Individual mechanism in activating of cell death was observed in each cell line of 6 melanomas.
Keywords: Specific gene expression prfiles after carbon ion irradiation
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| Overall design |
To study the gene expression profiles in human melanoma cell lines exposed to carbon ions or X-rays. We used 6 cell lines, 92-1, C32TG, Colo679, HMV-I, HMV-II, and MeWo. Cells were irradiated with 2Gy of carbon ions or X-rays, and total RNA was extracted. We used human whole genome array (55k).
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| Contributor(s) |
Matsumoto Y, Iwakawa M, Furusawa Y, Ishikawa K, Imadome K, Matsumoto I, Tsujii H, Ando K, Imai T |
| Citation(s) |
18386195 |
| Submission date |
Jan 24, 2008 |
| Last update date |
Oct 28, 2014 |
| Contact name |
Yoshitaka Matsumoto |
| E-mail(s) |
y_matsu@nirs.go.jp
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| Phone |
+81-43-206-3232
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| Fax |
+81-43-206-4149
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| Organization name |
National Institute of Radiological Sciences
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| Department |
Research Center for Charged Particle Therapy
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| Lab |
RadGenomics Research Group
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| Street address |
4-9-1, Anagawa, Inage-ku
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| City |
Chiba-shi |
| State/province |
Chiba |
| ZIP/Postal code |
263-8555 |
| Country |
Japan |
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| Platforms (1) |
| GPL2895 |
GE Healthcare/Amersham Biosciences CodeLink Human Whole Genome Bioarray |
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| Samples (108)
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| Relations |
| BioProject |
PRJNA98859 |
| Supplementary data files not provided |
| Processed data included within Sample table |
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