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Series GSE66303 Query DataSets for GSE66303
Status Public on Mar 31, 2016
Title Transcriptional responses to i.v. administered I-131 in various mouse normal tissues underlie diurnal variation
Organism Mus musculus
Experiment type Expression profiling by array
Summary Circadian rhythm study on transcriptional responses to i.v. administered 90 kBq iodine-131 after 24h in mouse kidney cortex and medulla, liver, lungs, spleen, and thyroid.
Female BALB/c nude mice (n=4/group) were i.v. injected with 90 kBq 131I at three different times of day, i.e. at 9.00 am, at 12.00 pm, or at 3.00 pm, and killed under anesthesia after 24h following treatment for excision of organs.
For each time of day, a control group (n=3-4/group) was i.v. injected with physiol. saline. The kidneys, liver, lungs, spleen, and thyroid were excised, flash-frozen, and stored at -80°C until extraction of total RNA.
Total RNA was extracted from homogenized tissue samples and subjected to expression analysis using RNA microarray technology.
 
Overall design The study consisted of 6 groups in total, i.e. 3 groups (n=4/group) that were i.v. injected with 90 kBq 131I (at 9.00 am, 12.00 pm, or 3.00 pm) and 3 sham-treated control groups (n=3-4/group) that were i.v. injected with physiol. saline (at 9.00 am, 12.00 pm, or 3.00 pm); please note that the number of control animals varied between 3-4 animals per group, i.e. n=4 treated at 9.00 am, n=3 treated at 12.00 pm, and n=3 treated at 3.00 pm. Six tissues were analyzed per animal, i.e. kidney cortex, kidney medulla, liver, lungs, spleen, and thyroid.
 
Contributor(s) Langen B, Rudqvist N, Parris TZ, Helou K, Forssell-Aronsson E
Citation(s) 26669694, 30001320
Submission date Feb 25, 2015
Last update date Jan 30, 2020
Contact name Britta Langen
E-mail(s) britta.langen@gu.se
Organization name Sahlgrenska Academy at University of Gothenburg
Department Department of Radiation Physics
Street address Gula stråket 2B
City Gothenburg
ZIP/Postal code SE-413 45
Country Sweden
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (136)
GSM1619245 Kidney cortex_90 kBq 131I i.v. injected at 9 am, excised after 24h_rep1
GSM1619246 Kidney cortex_90 kBq 131I i.v. injected at 9 am, excised after 24h_rep2
GSM1619247 Kidney cortex_90 kBq 131I i.v. injected at 9 am, excised after 24h_rep3
Relations
BioProject PRJNA276462

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66303_Non-normalized_data.txt.gz 20.2 Mb (ftp)(http) TXT
GSE66303_RAW.tar 3.1 Mb (http)(custom) TAR
Processed data included within Sample table
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