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| Status |
Public on Dec 31, 2015 |
| Title |
Gene expression profiling on C3H/10T1/2 fibroblasts during their myogenic conversion in case of Six4 ablation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
In this study, we used C3H/10T1/2 cells, a well known model of myogenic conversion, to study the effect of Six4 knockdown on the expression of genes during fibroblasts to myocytes conversion induced by ectopic expression of MyoD
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| Overall design |
We established C3H/10T1/2 cell line with stable Six4 knockdown by short hairpin RNA (shSix4) vs a control cell line (shLuc) and converted these cells into myogenic lineage by retroviral transduction of plasmid encoding Flag-MyoD-myc (pBABE-MyoD) or empty plasmid (pBABE). Cells were then induced to differentiate for 24 hours before RNA extraction.
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| Contributor(s) |
Chakroun I, Blais A |
| Citation(s) |
26229056 |
| Submission date |
Feb 26, 2015 |
| Last update date |
May 10, 2023 |
| Contact name |
Alexandre Blais |
| E-mail(s) |
alexandre.blais@uottawa.ca
|
| Organization name |
University of Ottawa
|
| Street address |
451 Smyth Road
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1H 8M5 |
| Country |
Canada |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA276526 |
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