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Series GSE66486 Query DataSets for GSE66486
Status Public on Apr 07, 2015
Title Response of IRF7-deficient peripheral blood mononuclear cells to pH1N1 influenza virus infection
Organism Homo sapiens
Experiment type Expression profiling by array
Summary IRF7 plays a critical role in the production and amplification of the antiviral type I and III interferon response. Autosomal recessive IRF7-deficiency resulted in life-threatening influenza disease in a 3-year-old child. We studied the impact of IRF7-deficiency in non-hematopoietic tissues (fibroblasts and lung epithelial cells) as well in hematopoietic cells (peripheral blood mononuclear cells (PBMCs)). Genome-wide gene expression analysis demonstrated a profound loss of type I and III IFNs in PBMCs infected with influenza virus.
 
Overall design PBMCs were isolated from 4 healthy donors and patients with deficiencies for IRF7 and UNC93B. The cells were infected with influenza virus A/CA/4/2009 at a multiplicity of infection (MOI) of 2 for 8 and 16 hours. Uninfected cells were cultured for 16 hours.
 
Contributor(s) Ciancanelli MJ, Huang SX, Luthra P, Garner H, Itan Y, Volpi S, Lafaille FG, Trouillet C, Schmolke M, Albrecht RA, Israelsson E, Lim H, Casadio M, Hermesh T, Lorenzo L, Leung LW, Pedergnana V, Boisson B, Okada S, Picard C, Ringuier B, Troussier F, Chaussabel D, Abel L, Pellier I, Notarangelo LD, Garcia-Sastre A, Basler CF, Geissmann F, Zhang S, Snoeck H, Casanova J
Citation(s) 25814066
Submission date Mar 03, 2015
Last update date Aug 13, 2018
Contact name Scott Presnell
E-mail(s) SPresnell@benaroyaresearch.org
Organization name Benaroya Research Institute
Department Systems Immunology
Street address 1201 Ninth Ave.
City Seattle
State/province WA
ZIP/Postal code 98101
Country USA
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (18)
GSM1623213 Ctrl1 NS
GSM1623214 Ctrl1 FLU_8h
GSM1623215 Ctrl1 FLU_16h
Relations
BioProject PRJNA277057

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66486_RAW.tar 26.2 Mb (http)(custom) TAR
GSE66486_non_normalized.txt.gz 5.7 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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