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| Status |
Public on Sep 01, 2015 |
| Title |
Transcriptome analysis of Dop1R2 RNAi in Drosophila melanogaster |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
The process of metamorphosis relies on choreography of temporal and spatial cues that mediate, in which inappropriate activation of many of these genes results in growth defects, developmental delay and death. Using RNA interference, we have unraveled an essential role for the insect specific dopamine 1(D1)-like receptor Dop1R2 at the late larval/prepupal stage. We used microarrays to evaluate differentially expressed genes during the early/pale pupal stage and identified up-regulation of families of functionally related genes, including ecdysone induced genes, stress and defense/immune response genes, and those that underlie tissue morphogenesis.
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| Overall design |
Drosophila Dop1R2 RNAi or w1118 control early/pale pupae were collected from crosses maintained at 29C. These tissues were processed for hybridization on Affymetrix microarrays (DrosGenome1). Results were used to assess differential expression of genes in Dop1R2 RNAi verses control flies.
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| Contributor(s) |
Regna K, Kurshan PT, Harwood BN, Jenkins AM, Lai CQ, Muskavitch MA, Kopin AS, Draper I |
| Citation(s) |
27184815 |
| Submission date |
Mar 04, 2015 |
| Last update date |
Oct 04, 2019 |
| Contact name |
Kimberly Regna |
| E-mail(s) |
kimberly.regna@gmail.com
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| Phone |
2019144525
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| Organization name |
Boston College
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| Department |
Biology
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| Lab |
Muskavitch
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| Street address |
140 Commonwealth Avenue
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| City |
Chestnut Hill |
| State/province |
Massachusetts |
| ZIP/Postal code |
02467 |
| Country |
USA |
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| Platforms (1) |
| GPL72 |
[DrosGenome1] Affymetrix Drosophila Genome Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA277148 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE66496_RAW.tar |
29.3 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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