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Series GSE6674 Query DataSets for GSE6674
Status Public on Dec 01, 2007
Title Gene expression program of AM-14 B cells stimulated through the B cell receptor (BCR) and/or Toll-like receptors (TLR)
Organism Mus musculus
Experiment type Expression profiling by array
Summary We have previously shown that rheumatoid factors (RF) produced by Fas-deficient autoimmune-prone mice typically bind autologous IgG2a with remarkably low affinity. Nevertheless, B cells representative of this RF population proliferate vigorously in response IgG2a/chromatin immune complexes through a mechanism dependent on the sequential engagement of the BCR and Toll-like receptor 9 (TLR9). To more precisely address the role of both receptors in this response, we analyzed the signaling pathways activated in AM14 B cells stimulated with these complexes. We found that the BCR not only serves to direct the chromatin complex to an internal compartment where it can engage TLR9 but also transmits a suboptimal signal that in combination with the signals emanating from TLR9 leads to NF?B activation and proliferation. Importantly, engagement of both receptors leads to the upregulation of a group of gene products, not induced by the BCR or TLR9 alone, that include IL-2. These data indicate that autoreactive B cells, stimulated by a combination of BCR and TLR9 ligands, acquire functional properties that may contribute to the activation of additional cells involved in the autoimmune disease process.
Keywords: cell stimulation
Overall design 15 Samples, 3 replicates for each treatment
Citation(s) 18025183
Submission date Jan 08, 2007
Last update date Feb 11, 2019
Contact name Jason W Bauer
Phone 612-625-5168
Organization name University of Minnesota
Street address 420 Deleware St SE MMC108
City Minneapolis
State/province MN
ZIP/Postal code 55455
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (15)
GSM154244 AM14 B cells, Media alone, rep1
GSM154245 AM14 B cells, Media alone, rep2
GSM154246 AM14 B cells, Media alone, rep3
BioProject PRJNA98613

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Supplementary file Size Download File type/resource
GSE6674_RAW.tar 90.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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