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| Status |
Public on Mar 14, 2015 |
| Title |
Transcriptional regulation by EVI1 in the absence or presence of TPA |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To investigate whether and how expression of the oncogenic transcription factor EVI1 influences gene regulation by phorbol esters and vice versa, the human myeloid cell line U937 was transduced with an EVI1 expression vector or empty vector as a control. Cells were treated with 12-Otetradecanoylphorbol 13-acetate (TPA) or its solvent ethanol as a control. RNA was extracted and subjected to gene expression microarray analysis.
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| Overall design |
U937 cells transduced with an EVI1 expression vector or empty vector as a control were treated with 50 ng/ml TPA or an equivalent amount of ethanol (solvent) for 24 h prior to gene expression microarray analysis. 3 biological replicates were performed.
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| Contributor(s) |
Steinmetz B, Hackl H, Wieser R |
| Citation(s) |
25486480 |
| Submission date |
Mar 13, 2015 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Rotraud Wieser |
| E-mail(s) |
rotraud.wieser@meduniwien.ac.at
|
| Organization name |
Medical University of Vienna
|
| Department |
Clinic of Medicine I
|
| Street address |
Waehringer Guertel 18-20
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA278114 |
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