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| Status |
Public on Mar 27, 2007 |
| Title |
MyD88-dependent changes in the pulmonary transcriptome after infection with Chlamydia pneumoniae |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Chlamydia pneumoniae, an obligate intracellular bacterium, causes pneumonia in humans and mice. Toll-like receptors and the key adaptor molecule MyD88 play a critical role in inducing immunity against this microorganism and are crucial to survive the infection. To explore the influence of MyD88 on induction of immune responses in vivo on a genome wide level, WT or MyD88-/- mice were infected with C. pneumoniae upon anesthesia and the pulmonary transcriptome was analyzed three days later by microarrays. We find that the infection induced the transcription of 360 genes and repressed 18 genes in WT mice. Of these, 221 genes were not or weakly induced in lungs of MyD88-/- mice. This cluster contains primarily genes encoding for chemokines, cytokines and other immune effector molecules. Genes induced by interferons were abundant in a cluster of 102 genes which were only partially MyD88-dependent. Interestingly, a set of 37 genes were induced more strongly in MyD88-/- mice and most of them are involved in the regulation of cellular replication. In summary, ex vivo analysis of the pulmonary transcriptome upon infection with C. pneumoniae demonstrated a major impact of MyD88 on inflammatory responses but not on interferon-type responses, and identified MyD88-independent genes involved in cellular replication
Keywords: lung, Chlamydia pneumoniae, mice, myd88, TLR, infection, knock out
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| Overall design |
Microarray analysis was performed in biological triplicates with RNA from individual mice derived from three independent infection experiments. Samples were lungs from wt and myd88 knock out mice, three days after Chlamydia pneumoniae or mock infection. Altogther 12 samples were analyzed on Affymetrix MOE430A arrays
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| Contributor(s) |
Mages J, RodrÃguez N, Dietrich H, Wantia N, Wagner H, Lang R, Miethke T |
| Citation(s) |
17374847 |
| Submission date |
Jan 09, 2007 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Joerg Mages |
| E-mail(s) |
mages@lrz.tum.de
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| Organization name |
Technical University Munich
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| Department |
Institute of Medical Microbiology, Immunology and Hygiene
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| Street address |
Trogerstrasse 30
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| City |
Munich |
| ZIP/Postal code |
81675 |
| Country |
Germany |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA99067 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE6688_RAW.tar |
42.8 Mb |
(http)(custom) |
TAR (of CEL) |
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