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| Status |
Public on Mar 27, 2007 |
| Title |
ANA-1 macrophages infected with Chlamydia pneumoniae |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
This experiment is an additional experiment to GSE6688. Mouse macrophages (ANA-1 cells) were infected in vitro with C. pneumoniae with a M.O.I. of 10. Twenty two genes were significantly upregulated. Examples of the most upregulated genes in mouse macrophages after C. pneumoniae infection are serum amyloid A3 (saa3), a protein that is mainly produced by activated macrophages during tissue injury or inflammation, MIP-2 (cxcl2) and irg1. Expression levels of all genes induced by C. pneumoniae in macrophages in vitro correlated with the results obtained from infected lungs from wild type mice (GSE6688), suggesting that this cell type participates in host defense in vivo against C. pneumoniae.
Keywords: Chlamydia pneumoniae, ANA-1 macrophages, in vitro, infection
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| |
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| Overall design |
ANA-1 macrophages were infected with Chlamydia pneumoniae or left untreated. After 8h total RNA was extracted. Two biological replicates were performed resulting in 4 arrays in total
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| |
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| Contributor(s) |
Mages J, RodrÃguez N, Dietrich H, Wantia N, Wagner H, Lang R, Miethke T |
| Citation(s) |
17374847 |
| Submission date |
Jan 09, 2007 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Joerg Mages |
| E-mail(s) |
mages@lrz.tum.de
|
| Organization name |
Technical University Munich
|
| Department |
Institute of Medical Microbiology, Immunology and Hygiene
|
| Street address |
Trogerstrasse 30
|
| City |
Munich |
| ZIP/Postal code |
81675 |
| Country |
Germany |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA99071 |
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