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| Status |
Public on Mar 18, 2018 |
| Title |
Splicing events and transcriptome changes upon TDP-43 and FUS knock-down in human neuroblastoma cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We performed exon array analysis to define and compare the transcriptomic changes, in terms of splicing and gene expression profilings, occurring in human neuroblastoma cells upon knocking down TDP-43 and FUS RNA-binding proteins (RBP), with the final aim to unravel the biological role of these RBPs in neuronal cells.
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| Overall design |
To identify the differentially expressed and spliced genes, the microarray-based technology was performed using the recovered total RNAs from triplicate experimental knocked-down samples (siTDP-43 samples, siFUS samples and the control mock siCTRL samples).
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| Contributor(s) |
Ratti A, de la Grange P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Mar 18, 2015 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Pierre de la Grange |
| E-mail(s) |
pierre.delagrange@genosplice.com
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| Organization name |
GenoSplice technology
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| Department |
Paris Biotech Santé
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| Street address |
29, rue du Faubourg Saint-Jacques
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| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
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| Platforms (2) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
| GPL5188 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [probe set (exon) version] |
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| Samples (18)
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| GSM1636383 |
control-silenced SK-N-BE cells, biological rep 1 (transcription-level) |
| GSM1636384 |
control-silenced SK-N-BE cells, biological rep 2 (transcription-level) |
| GSM1636385 |
control-silenced SK-N-BE cells, biological rep 3 (transcription-level) |
| GSM1636386 |
TDP-43-silenced SK-N-BE cells, biological rep 1 (transcription-level) |
| GSM1636387 |
TDP-43-silenced SK-N-BE cells, biological rep 2 (transcription-level) |
| GSM1636388 |
TDP-43-silenced SK-N-BE cells, biological rep 3 (transcription-level) |
| GSM1636389 |
FUS-silenced SK-N-BE cells, biological rep 1 (transcription-level) |
| GSM1636390 |
FUS-silenced SK-N-BE cells, biological rep 2 (transcription-level) |
| GSM1636391 |
FUS-silenced SK-N-BE cells, biological rep 3 (transcription-level) |
| GSM1636392 |
control-silenced SK-N-BE cells, biological rep 1 (exon-level) |
| GSM1636393 |
control-silenced SK-N-BE cells, biological rep 2 (exon-level) |
| GSM1636394 |
control-silenced SK-N-BE cells, biological rep 3 (exon-level) |
| GSM1636395 |
TDP-43-silenced SK-N-BE cells, biological rep 1 (exon-level)) |
| GSM1636396 |
TDP-43-silenced SK-N-BE cells, biological rep 2 (exon-level) |
| GSM1636397 |
TDP-43-silenced SK-N-BE cells, biological rep 3 (exon-level) |
| GSM1636398 |
FUS-silenced SK-N-BE cells, biological rep 1 (exon-level) |
| GSM1636399 |
FUS-silenced SK-N-BE cells, biological rep 2 (exon-level) |
| GSM1636400 |
FUS-silenced SK-N-BE cells, biological rep 3 (exon-level) |
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| Relations |
| BioProject |
PRJNA278758 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE67027_RAW.tar |
417.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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