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Series GSE67134 Query DataSets for GSE67134
Status Public on Mar 21, 2015
Title Expression data from KSL and GMP cells of FLT3/ITD and FLT3/ITD-SmoM2 mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary FLT3/ITD-SmoM2 mice developed rapidly fatal myeloid leukemia compared to FLT3/ITD only mice, suggesting that overactivation of the Hedgehog signaling pathway via SmoM2 can drive myeloid disease progression
We used the Affymetrix Mouse 430_2.0 microarray to detail global gene expression responsible for disease progression in sorted bone marrow cells and found that the Hedgehog signaling pathway contributes to disease progression by enhancing FLT3 signaling
 
Overall design KSL and GMP cells were FACS sorted from wild-type, FLT3/ITD and FLT3/ITD-SmoM2 bone marrow when the mice were around 3 months of age, when the FLT3/ITD-SmoM2 mice developed leukemia. RNA was extracted from the sorted cells and hybridized onto the Mouse 430_2.0 Affymetrix array. Wild-type and FLT3/ITD mice that were used as controls were littermates of the FLT3/ITD-SmoM2 leukemic mice. Each sample consists of pooled cells from 3 mice.
 
Contributor(s) Lim Y, Matsui W
Citation(s) 26062848
Submission date Mar 20, 2015
Last update date Feb 11, 2019
Contact name Yiting Lim
Organization name Johns Hopkins University School of Medicine
Street address 1650 Orleans Street
City Baltimore
State/province MD
ZIP/Postal code 21287
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM1639867 GMP cells from wild type mouse bone marrow
GSM1639868 KSL cells from wild type bone marrow
GSM1639869 GMP cells from FLT3/ITD mouse bone marrow
Relations
BioProject PRJNA279028

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67134_RAW.tar 24.6 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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