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| Status |
Public on Feb 09, 2017 |
| Title |
Expression data from intravaginal Chlamydia-infected mouse T lymphocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Intravaginal infection of C57BL/6 mice with Chlamydia muridarum promotes activation and migration of effector lymphocytes to the genital tract mucosa. Among up regulated genes related to the specific immune response 7 days after infection we aimed to detect adhesion molecules associated with migration to this mucosa.
We used microarrays to determine the global gene expression changes underlying homing to genital tract mucosa and identified distinct classes of up-regulated genes during effector T lymphocyte migration to this tissue.
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| Overall design |
Circulating TEM cells were sorted from three 7d Chlamydia-infected mice (VAG group) and three contemporary sham-treated mice (controls). Blood samples (≈500µl) were immediately lysed using a RBC lysis buffer. T cells were identified as CD3+ and finally sorted as CD3+ CD62L- CD44+ activated T effector memory cells. Total RNA from each of the six samples was analyzed with an individual Affymetrix HT MG-430 PM Strip Array.
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| Contributor(s) |
Qualai J, Li L, Carrascosa JM, Cabré E, Falguera G, Sumoy L, McSorley SJ, Genesca M |
| Citation(s) |
27272720 |
| Submission date |
Mar 23, 2015 |
| Last update date |
Aug 06, 2018 |
| Contact name |
Lauro Sumoy |
| E-mail(s) |
lsumoy@igtp.cat
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| Organization name |
IGTP
|
| Department |
High Content Genomics and Bioinformatics
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| Street address |
Ctra. Can Ruti, Camí de les escoles s/n
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| City |
Badalona |
| State/province |
Barcelona |
| ZIP/Postal code |
08916 |
| Country |
Spain |
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| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA279122 |
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