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| Status |
Public on Mar 31, 2015 |
| Title |
The basic helix-loop-helix transcription factor Mist1 overexpression in mouse fetal hepatoblast culture |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To identify the molecular mechanism of Mist1 during hepatoblasts differentiation, we performed gene expression analysis in Mist1 over-expressed cells. Dlk+ hepatoblasts derived from E13 fetal livers were purified. Purification of Dlk+ cells were performed using anti-Dlk antibody after hematopoietic cells were removed. Cells were infected with mock or Mist1-overexpressing retroviruses and cultured for 3days with oncostatin M.
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| Overall design |
Virus infection was performed at the day primary culture was began (day0). Oncostatin M was added at day 1 and the cells were collected at day3. Two independent experiments were performed.
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| Contributor(s) |
Chikada H, Kamiya A |
| Citation(s) |
26456005 |
| Submission date |
Mar 30, 2015 |
| Last update date |
Jul 29, 2019 |
| Contact name |
Akihide Kamiya |
| E-mail(s) |
kamiyaa@tokai-u.jp
|
| Organization name |
Tokai University
|
| Street address |
143 Shimokasuya
|
| City |
Isehara |
| State/province |
Kanagawa |
| ZIP/Postal code |
259-1193 |
| Country |
Japan |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA279839 |
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