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Series GSE67523 Query DataSets for GSE67523
Status Public on Jan 03, 2017
Title Biological characterization of gene response to insulin-induced hypoglycemia in mouse retina
Organism Mus musculus
Experiment type Expression profiling by array
Summary Glucose is the most important metabolic substrate of the retina and maintenance of nor-moglycemia is an essential challenge for diabetic patients. Glycemic excursions could lead to cardiovascular disease, nephropathy, neuropathy and retinopathy. We recently showed that hy-poglycemia induced retinal cell death in mouse via caspase 3 activation and glutathione (GSH) decrease. Ex vivo experiments in 661W photoreceptor cells confirmed the low-glucose induction of death via superoxide production and activation of caspase 3, which was concomitant with a decrease of GSH content. We evaluate herein retinal gene expression 4 h and 48 h after insulin-induced hypoglycemia. Microarray analysis demonstrated clusters of genes whose expression is modified by hypoglycemia and we discuss the potential implication of those genes in retinal cell death. In addition, we highlight, by gene set enrichment analysis, three important pathways, including KEGG lysosomes, KEGG GSH metabolism and REACTOME apoptosis pathways. We tested the effect of recurrent hypoglycemia (three successive 5h periods of hypoglycemia separated by 48 h recovery) on retinal cell death. Interestingly, exposure to multiple hypoglycemic events prevents retinal cell death and GSH decrease, or adapts the retina to external stress by restoring GSH level comparable to control situation. We hypothesize that scavenger GSH is a key compound in this apoptotic process, and maintaining “normal” GSH level, as well as a strict glycemic control, may represent a therapeutic challenge in order to avoid side effects of diabetes, especially diabetic retinopathy.
 
Overall design 4 experimental groups, 3 samples per group
 
Contributor(s) Emery M, Nanchen N, Ibberson M, Preitner F, Thorens B, Schorderet DF, Roduit R
Citation(s) 26918849
Submission date Apr 02, 2015
Last update date Jan 04, 2017
Contact name Mark Ibberson
Organization name SIB Swiss Institute of Bioinformatics
Department Vital-IT
Street address Genopode building
City Lausanne
ZIP/Postal code CH-1015
Country Switzerland
 
Platforms (1)
GPL10740 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [probe set (exon) version]
Samples (12)
GSM1648752 Retina, 4 hours, hypoglycemic clamp, replicate 1
GSM1648753 Retina, 4 hours, hypoglycemic clamp, replicate 2
GSM1648754 Retina, 4 hours, hypoglycemic clamp, replicate 3
Relations
BioProject PRJNA280156

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Supplementary file Size Download File type/resource
GSE67523_RAW.tar 48.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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