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| Status |
Public on Mar 22, 2007 |
| Title |
Localization and mechanism of S. pombe histone demethylases |
| Platform organism |
Schizosaccharomyces pombe 972h- |
| Sample organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by genome tiling array
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
We profiled the genomic localization of two histone demethylases, LSD1 and LSD2 using ChIP-on-chip. Deletions in LSD2 are lethal but not in LSD1, so we examined effect of an lsd1 deletion on genomewide expression levels and localization patterns of H3me2K4 and H3me2K9 relative to wild-type.
Keywords: mutant vs wild-type
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| Overall design |
Each experiment (transcription or ChIP localization) was implemented as a reference design including a dye reversal. Additional statistical power is added by the fact that the all experimental spots on the array platform are printed in duplicate.
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| Contributor(s) |
Lan F, Zaratiegui M, Villen J, Vaughn MW, Verdel A, Huarte M, Shi Y, Gygi S, Moazed D, Martienssen RA, Shi Y |
| Citation(s) |
17434129 |
| Submission date |
Jan 16, 2007 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Matthew Wayne Vaughn |
| E-mail(s) |
vaughn@tacc.utexas.edu
|
| Phone |
(512) 232-7124
|
| Organization name |
University of Texas at Austin
|
| Department |
Texas Advanced Computing Center
|
| Lab |
Vaughn
|
| Street address |
10100 Burnet Rd
|
| City |
Austin |
| State/province |
TX |
| ZIP/Postal code |
78758 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL4749 |
CSHL S. pombe Genomic Tiling Microarray |
|
| Samples (7)
|
| GSM155375 |
ChIP-chip localization of S. pombe LSD1 histone demethylase |
| GSM155377 |
ChIP-chip localization of S. pombe LSD2 histone demethylase |
| GSM155390 |
Transcriptional profilng of the S. pombe histone demethylase mutant lsd1 |
| GSM155546 |
ChIP-chip examination of H3me2K9 in S. pombe wild-type |
| GSM155547 |
ChIP-chip examination of H3me2K9 in S. pombe histone demethylase mutant lsd1 |
| GSM155548 |
ChIP-chip examination of H3me2K4 in S. pombe wild-type |
| GSM155549 |
ChIP-chip examination of H3me2K4 in S. pombe histone demethylase mutant lsd1 |
|
| Relations |
| BioProject |
PRJNA98947 |
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