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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 05, 2016 |
| Title |
CARD9 impacts colitis by altering gut microbiota metabolism of tryptophan into aryl hydrocarbon receptor ligands |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
It is crucial to decipher the host-microbiota interactions as they are involved in intestinal homeostasis and diseases. Caspase Recruitment Domain 9 (Card9) is an inflammatory bowel disease (IBD) susceptibility gene coding for an adapter protein for innate immunity toward many microorganisms. Card9 mediates colitis recovery via interleukin 22 pathway activation and Card9-/- mice have enhanced susceptibility to colitis. To reveal the mechanisms responsible of this defect in Card9-/-mice, we compared colon transcriptomics in WT and Card9-/- mice before and during DSS-induced colitis. Mice transcriptomes clusterized according to the genotype supporting a pattern clearly different in WT and Card9-/- mice. The number of up-regulated genes at day 7 was largely higher in Card9-/- compared to WT mice. Pathway analyses of the induced transcripts showed a dominance of immune-related pathway with a stronger signal in Card9-/- mice. Interestingly, NOD-like receptor signaling pathway, in which CARD9 is involved, was an exception with weaker activation in Card9-/- than in WT mice. During the recovery period at day 12, pathways involved in cell proliferation and replication were significantly activated in WT compared to Card9-/- mice confirming the healing defect in Card9-/- mice. Results published in Nature Medicine, doi:10.1038/nm.4102
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| Overall design |
For the induction of colitis, mice were given drinking water supplemented with 2% (w/v) Dextran sulphate sodium (DSS) for 7 days, then allowed to recover by drinking water alone for 5 additional days. 3 mice of each groups (WT and Card9-/-) were sacrified before DSS administration. 5 WT mice and 5 Card9-/- mice were sacrified 7 days after DSS administration and 5 mice of each group were sacrified at day 12.
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| Contributor(s) |
Lamas B, Richard ML, Leducq V, Pham H, Michel M, Da Costa G, Bridonneau C, Jegou S, Hoffmann TW, Natividad JM, Brot L, Taleb S, Couturier-Maillard A, Nion-Larmurier I, Merabtene F, Seksik P, Bourrier A, Cosnes J, Ryffel B, Beaugerie L, Launay J, Langella P, Xavier RJ, Sokol H |
| Citation(s) |
27158904 |
| Submission date |
Apr 03, 2015 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Bruno LAMAS |
| E-mail(s) |
lamasbruno@hotmail.fr
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| Organization name |
Unité INSERM 1157/UMR 7203
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| Department |
CHU St Antoine
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| Lab |
Avenir Team Gut Microbiota and Immunity
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| Street address |
27 rue Chaligny
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| City |
Paris |
| ZIP/Postal code |
75012 |
| Country |
France |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (25)
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| Relations |
| BioProject |
PRJNA280289 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE67577_RAW.tar |
299.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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