|
| Status |
Public on Apr 08, 2015 |
| Title |
Genome-wide analysis of Smad2 and FoxH1 binding sites in zebrafish blastula |
| Organism |
Danio rerio |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Morphogen gradients expose cells to different signal concentrations and induce target genes with different ranges of expression. To determine how the Nodal morphogen gradient induces distinct gene expression patterns during zebrafish embryogenesis we characterized the binding sites of the Nodal-specific transcription co-activators Smad2 and FoxH1 in wild-type, Nodal-overexpressing or Nodal signaling inhibited embryos. We found that FoxH1 binds to Nodal targets loci even in the absence of the signal, while Smad2 only binds and colocalizes to FoxH1 upon induction of the Nodal pathway.
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| |
|
| Overall design |
6 samples total.
|
| |
|
| Contributor(s) |
Akhmetova L, Dubrulle J, Schier AF |
| Citation(s) |
25869585 |
| Submission date |
Apr 07, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Julien Dubrulle |
| E-mail(s) |
dubrullejulien@gmail.com
|
| Phone |
281-508-9180
|
| Organization name |
Harvard University
|
| Department |
MCB
|
| Lab |
Schier
|
| Street address |
16 Divinity Ave
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA280533 |
| SRA |
SRP056934 |
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