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| Status |
Public on Apr 10, 2015 |
| Title |
Gene Array Data from p0 Mouse pup; ascending and descending aortas; fibulin-4 knockout and control |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Fibulin-4 plays an essential role in elastic fiber formation, though it's exact function is unclear. Mice lacking the fibulin-4 gene develop cutis laxa with thoracic aortic aneurysms and have narrowed descending aortic diamaters, dying shortly after birth. Another model that disrupt elastic fiber formation, elastin gene knockeds, are also perinatally lethal and have narrowed descending aortas but do not develop thoracic aneurysms.
We hypothesized that there may be altered gene expression to explain the altered anatomy based on aortic tissue location we observed, which may provide therapeutic target(s)
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| Overall design |
Ascending and descending aortas of p0 mouse pups were dissected, pooled in groups of eight, and homogenized to isolate RNA and we used microarrays on the pooled samples to identify genes that had expression significantly changed.
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| Contributor(s) |
Procknow J, Wagenseil J |
| Citation(s) |
25934097 |
| Submission date |
Apr 09, 2015 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Jessica Wagenseil |
| E-mail(s) |
jessica.wagenseil@wustl.edu
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| Phone |
(314) 935-5052
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| Organization name |
Washington University
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| Department |
Mechanical Engineering and Materials Sciences
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| Street address |
1 Brookings Dr. Box 1185
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| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63130 |
| Country |
USA |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (8)
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| GSM1654638 |
Ascending aorta from wild-type pup, biological replicate 1 |
| GSM1654639 |
Ascending aorta from wild-type pup, biological replicate 2 |
| GSM1654640 |
Descending aorta from wild-type pup, biological replicate 1 |
| GSM1654641 |
Descending aorta from wild-type pup, biological replicate 2 |
| GSM1654642 |
Ascending aorta from fibulin-4 knockout pup, biological replicate 1 |
| GSM1654643 |
Ascending aorta from fibulin-4 knockout pup, biological replicate 2 |
| GSM1654644 |
Descending aorta from fibulin-4 knockout pup, biological replicate 1 |
| GSM1654645 |
Descending aorta from fibulin-4 knockout pup, biological replicate 2 |
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| Relations |
| BioProject |
PRJNA280736 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE67707_RAW.tar |
69.0 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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