|
| Status |
Public on Feb 19, 2016 |
| Title |
Profiling of soma and neurite transcriptomes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report mRNA profiles of subcellularly localized transcriptomes (soma and neurite) of two mouse cell lines, N2A and CAD, as well as primary cortical neurons from E18.5 mice. We also performed this fractionation and sequencing after RNAi knockdown (cell lines) or in knockout mice (primary cortical neurons) of the RNA-binding proteins muscleblind 1 and 2 (Mbnl1 and Mbnl2).
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| |
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| Overall design |
Fractionate neurons using porous transwell membranes. Isolate poly-A RNA.
|
| |
|
| Contributor(s) |
Taliaferro M, Burge C |
| Citation(s) |
26907613 |
| Submission date |
Apr 13, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Matthew Taliaferro |
| Organization name |
University of Colorado Anschutz Medical Campus
|
| Department |
Biology
|
| Street address |
12801 E 17th Ave, RC1 South Room 10403D
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (48)
|
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| Relations |
| BioProject |
PRJNA281008 |
| SRA |
SRP057123 |
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