|
| Status |
Public on Dec 07, 2015 |
| Title |
Genome-wide maps of BAF180 and REV-ERBα binding |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We generated genome-wide cistromes of BAF180 subunit of the SWI-SNF chromatin remodeling complex in mouse liver at CT10 and CT22. In addition, we performed ChIP-Seq analysis on REV-ERBα in WT and SRC-2-/- mouse liver at CT10. We found circadian oscilation of BAF180 chromatin recruitment in mouse liver with peak recruitment at CT22 and nadir at CT10. Further,REV-ERBα chromatin recruitment was significantly reduced in SRC-2-/- mouse liver compared to WT mice at CT10.
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| Overall design |
ChIP-Seq for BAF180 was performed in WT mice liver at CT10 and CT22 using two different antibodies. ChIP-Seq for REV-ERBα was performed in WT and SRC-2-/- mice in liver at CT10 with biological replicates.
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| |
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| Contributor(s) |
Zhu B, York B, O'Malley BW |
| Citation(s) |
26611104, 29615789 |
| Submission date |
Apr 14, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Bokai Zhu |
| E-mail(s) |
bzhu@bcm.edu
|
| Phone |
2192085319
|
| Organization name |
Baylor College of Medicine
|
| Department |
MCB
|
| Lab |
Post-doc
|
| Street address |
One Baylor Plaza, M813
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE67860 |
Coactivator-Dependent Oscillation of Chromatin Accessibility Dictates Circadian Gene Amplitude through REV-ERB Loading |
|
| Relations |
| BioProject |
PRJNA281076 |
| SRA |
SRP057150 |
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