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| Status |
Public on May 08, 2015 |
| Title |
Investigation about fibroblasts of different origins in culture |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of this study was to determine if fibroblasts from different origin (skin, colon, tumors) were keeping their characteristic while extracted and cultured ex vivo for several passages. HUVEC was used as a control, being cells from a different background. Surprisingly, fibroblasts from different origins are losing their independant characteristic to cluster in a similar way after 5-6 passages in culture in vitro, showing an activated status.
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| Overall design |
Fibroblasts were extracted from human skin, colon normal stroma and colon tumor stroma. HUVECs were extracted from human samples at the same time. All cells, each group from 3 different patients, were grown on plastic for 5 passages and mRNA was extracted to perform RNASeq analysis.
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| |
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| Contributor(s) |
Rüegg C, Knuchel S, Anderle P |
| Citation(s) |
25973543 |
| Submission date |
Apr 16, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Rüegg Curzio |
| E-mail(s) |
curzio.ruegg@unifr.ch
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| Organization name |
Université de Fribourg
|
| Department |
Département de Médecine 3
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| Lab |
Pathology
|
| Street address |
Albert-Gockel 1
|
| City |
Fribourg |
| ZIP/Postal code |
1700 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA281358 |
| SRA |
SRP057251 |
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