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Series GSE6803 Query DataSets for GSE6803
Status Public on Sep 17, 2008
Title Effects of Leuzea carthamoides (maral root) in MCF-7 cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Products derived from roots of Leuzea carthamoides DC. (maral root) are being promoted as anti-aging and adaptogenic. The phytoecdysteroids are considered as active principles with numerous beneficial effects, but little is known about the pharmacological properties of Leuzea extracts. We, therefore, investigated the effects of a lipophilic Leuzea root extract on ER+ breast cancer MCF-7 cells at transcriptional level in comparison to 17beta-estradiol and the ER antagonist tamoxifen. With the extract 241 genes were regulated more than 1.5 fold. We observed gene regulation in an anti-proliferative and pro-apoptotic manner.
Additionally, expression of several enzymes with oxidoreductase activity was induced including a very strong increase of the phase I enzyme CYP1A1, a possible link to the AhR pathway, which might explain other expression results, e.g. the correlated regulation of about 20 genes by Leuzea compared to 17beta-estradiol.
Keywords: treatment with Leuzea carthamoides
 
Overall design MCF-7 cells were treated for 24 h with a lipophilic (dichloromethane) Leuzea root extract, 17beta-estradiol, tamoxifen and the solvent control (0,1% DMSO) in duplicate
 
Contributor(s) Gaube F, Wolfl S, Pusch L, Kroll TC, Hamburger M
Citation(s) 18975255
Submission date Jan 19, 2007
Last update date Mar 25, 2019
Contact name Friedemann Gaube
E-mail(s) Friedemann.Gaube@uni-jena.de
Phone +49 3641 949844
Fax +49 3641 949842
URL http://www.uni-jena.de/Pharmazeutische_Biologie.html
Organization name Friedrich-Schiller-University Jena
Department Pharmacy
Lab Pharmaceutical Biology
Street address Semmelweisstr. 10
City Jena
ZIP/Postal code 07743
Country Germany
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (8)
GSM155006 24h_DMSO_1
GSM155007 24h_DMSO_2
GSM155008 24h_Estradiol_1
Relations
BioProject PRJNA99203

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6803_RAW.tar 73.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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