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Series GSE68056 Query DataSets for GSE68056
Status Public on Mar 07, 2016
Title Transcriptional profiling of antigen-specific CD8 T cells from wildtype and mutant mice.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To understand CD8 effector T cell differentiation in more detial we have used transcriptional profiling of antigen-specific CD8 T cells deficient in Blimp1, IL-2ra, or both, or Tbet. We reveal a common program of effector differentiation regulated by cytokine signaling and the combined activities of Blimp1 and T-bet, indicating remarkable redundancy and specificity in the control of genes involved in the differentiation of effector T cells.
 
Overall design Bone marrow chimeric mice were generated containing congenically marked wildtype and mutant heamatopoietic cells. The mice were infected with primed with PR8 influenza virus. Six weeks later they were infected with the heterologous HKx31 influenza virus. Antigen-specific (NP366) positive CD8 T cells were sorted. RNA was exracted and RNA sequening performed.
 
Contributor(s) Xin A, Kallies A, Leonard W, Shi W, Liao Y, Smyth G
Citation(s) 26950239
Submission date Apr 20, 2015
Last update date May 15, 2019
Contact name Wei Shi
E-mail(s) Wei.Shi@onjcri.org.au
Organization name Olivia Newton John Cancer Research Institute
Department Bioinformatics and Cancer Genomics
Street address Level 5, ONJ Cancer Centre, 145 Studley Rd
City Heidelberg
State/province VIC
ZIP/Postal code 3084
Country Australia
 
Platforms (1)
GPL9185 Illumina Genome Analyzer (Mus musculus)
Samples (16)
GSM1661817 WT_075
GSM1661818 WT_077
GSM1661819 WT_079
Relations
BioProject PRJNA281635
SRA SRP057459

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68056_RAW.tar 5.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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