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Series GSE6810 Query DataSets for GSE6810
Status Public on Aug 08, 2007
Title Response of murine splenocytes to infection with wild type or virB mutant Brucella strains
Organism Mus musculus
Experiment type Expression profiling by array
Summary The virB operon, encoding a Type IV secretion system (T4SS), is essential for intracellular survival and persistent infection of Brucella spp. To better understand the role of the T4SS in evading host defense mechanisms and establishing chronic infection, we compared transcriptional profiles of the host response to infection with wild type Brucella strains and strains that fail to express the virB genes. Analysis of host gene expression profiles three days after inoculation with wild type Brucella strains revealed an inflammatory response dominated by interferon-induced genes. This analysis found that not only the type II but also type I interferon pathway was elicited by Brucella infection. Real time RT-PCR showed that a group of genes from these pathways was induced by day 3 post-infection and declined to baseline levels by day 7. In contrast, neither of the two virB mutant strains elicited expression of interferon-induced genes, demonstrating that the T4SS was required to trigger an inflammatory response early during infection.
Keywords: analysis of transcriptional responses induced by infection
 
Overall design We performed microarray-based expression analyses of splenocytes from mice infected with two virulent strains (B. abortus 2308 and B. melitensis 16M), and two different B. abortus virB mutants, whose virB operon was either disrupted (BA41) or completely deleted (ADH4.2), to better understand the contribution of the T4SS in establishing infection of the reticuloendothelial system. 3 days after infection of mice, spleens were excised for RNA extraction. For each bacterial strain, RNA from 5 mice was pooled and reverse transcribed for hybridization to an array. Each experiment was performed in duplicate.
 
Contributor(s) Roux CM, Tsolis RM
Citation(s) 17441987
Submission date Jan 19, 2007
Last update date Feb 11, 2019
Contact name Renee Tsolis
E-mail(s) rmtsolis@ucdavis.edu
Organization name University of California at Davis
Department Medical Microbiology & Immunology
Lab Tsolis
Street address One Shields Avenue
City Davis
State/province CA
ZIP/Postal code 95616
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (10)
GSM155205 Control replicate 1
GSM155248 Control replicate 2
GSM155249 B. abortus 2308 replicate 1
Relations
BioProject PRJNA99215

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Supplementary file Size Download File type/resource
GSE6810_RAW.tar 58.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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