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Series GSE6813 Query DataSets for GSE6813
Status Public on Dec 19, 2008
Title Gene expression profiles of CD4+CD25+ Tregs from NOD and B6.H2g7 mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary The NOD (nonobese diabetic) mouse strain develops a characteristic autoimmune syndrome that closely resembles human type I diabetes. It has been suggested that NOD mice exhibit both numerical deficiency in CD4+CD25+ regulatory T cells (Treg) and reduced suppressive activity. We compared sorted CD4+CD25+ Tregs from the spleens of 6-7 week-old female NOD and nondiabetic B6.H2g7 mice. Tregs were 93±2% and 95±1% Foxp3+ in NOD and B6.H2g7 cells, respectively, on post-sort reanalysis. "Conventional" CD4+CD25- T cells (Tconv) are included as reference populations. Surprisingly, Treg "signature" is similar between the two strains, with only a few probesets that subtly deviate.
Keywords: Cell population comparison from two mouse strains.
 
Overall design For each strain (NOD and B6.g7), we analyzed two populations: CD4+CD25+ Treg and CD4+CD25- Tconv cells, for a total of four distinct populations. RNA from three mice were pooled for each replicate; there are three independent replicates for each population. After RMA normalization, intensity values were averaged across the three replicates and analyzed. We calculated the ratio of Treg/Tconv intensity values for each strain and compared the results.
 
Contributor(s) Auyeung VC, Feuerer M, Hill J, Holler P, Jiang W, Campbell C, Mathis D, Benoist C
Citation(s) 19073938
Submission date Jan 20, 2007
Last update date Feb 11, 2019
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM155600 CD4+CD25- Tconv, B6.H2g7, rep1
GSM155632 CD4+CD25- Tconv, B6.H2g7, rep2
GSM155633 CD4+CD25- Tconv, B6.H2g7, rep3
Relations
BioProject PRJNA99189

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Supplementary file Size Download File type/resource
GSE6813_RAW.tar 45.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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