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Series GSE6821 Query DataSets for GSE6821
Status Public on Jun 01, 2007
Title Global gene expression analysis of GMP from Icsbp (Irf-8) deficient mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary In order to study the consequences of the loss of Icsbp expression in hematopoiesis Granulocyte-Monocyte Progenitors from bone marrow were isolated from Icsbp wild type and deficient mice by flow cytometry. Global gene expression was performed using Affymetrix gene chip technology.
Keywords: Gene modification, Irf-8, knock out mice
 
Overall design GMP from Icsbp wild type and deficient mice were isolated as described by Terszowski et al. (Blood. 2005 Mar 1;105(5):1937-45). Wild type GMP were prepared from two independent biological replica using pooled bone marrow from alt least 10 independent mice. The first was analyzed using the Affymetrix MOE 430A and the second using MOE430_2. GMP from Icsbp deficient mice were prepared from three independent biological replica from at least 8 individual mice. The first two samples were analyzed using the Affymetrix MOE 430A and the third using MOE430_2.
 
Contributor(s) Lenze D, Koenigsmann J, Rodewald HR, Terszowski G, Carstanjen D
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Submission date Jan 22, 2007
Last update date Feb 11, 2019
Contact name Dirk Carstanjen
E-mail(s) carstanjen@fmp-berlin.de
Phone +49-30-8371-1917/ -1911
Fax +49-30-8371-1922
URL http://www.fmp-berlin.de
Organization name Institute for Molecular Pharmacology
Department Molecular Genetics
Lab Molecular Myelopoiesis
Street address Krahmer Strasse 6
City Berlin-Steglitz
State/province Berlin
ZIP/Postal code 12207
Country Germany
 
Platforms (2)
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (4)
GSM27216 GMP001
GSM157222 GMPho_02
GSM157223 GMPho_430_2
Relations
BioProject PRJNA99177

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6821_RAW.tar 14.1 Mb (http)(custom) TAR (of CEL, EXP)
Raw data provided as supplementary file

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