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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 01, 2007 |
| Title |
Transcripts targeted by the microRNA-16 family cooperatively regulate cell cycle progression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
microRNAs (miRNAs) are abundant, ~21 nucleotide (nt) non-coding regulatory RNAs. Each miRNA may regulate hundreds of mRNA targets, but the identities of these targets and the processes they regulate are poorly understood. Here we have explored the use of microarray profiling and functional screening to identify targets and biological processes triggered by transfection of miRNAs into human cells. We demonstrate that a family of miRNAs sharing sequence identity with miR-16 negatively regulates cellular growth and cell cycle progression. miR-16 down-regulated transcripts were enriched for genes whose silencing by siRNAs causes cell cycle accumulation at G0/G1. Simultaneous silencing of these genes was more effective at blocking cell cycle progression than disruption of the individual genes. Thus, miR-16 coordinately regulates targets that may act in concert to control cell cycle progression.
Keywords: miRNA, microRNA, miR-16, target, cell cycle, G0/G1, microarray profiling, functional screen,
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| Overall design |
Please see for details: Linsley PS, Schelter J, Burchard J, Kibukawa M, Martin MM, Bartz SR, Johnson JM, Cummins JM, Raymond CK, Dai H, Chau N, Cleary M, Jackson AL, Carleton M, and Lim L. MCB (2007).
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| Contributor(s) |
Linsley PS, Schelter J, Burchard J, Kibukawa M, Martin MM, Bartz SR, Johnson JM, Cummins JM, Raymond CK, Dai H, Chau N, Cleary M, Jackson AL, Carleton ML, Lim L |
| Citation(s) |
17242205 |
| Submission date |
Jan 22, 2007 |
| Last update date |
Nov 05, 2014 |
| Contact name |
Julja Burchard |
| Organization name |
Merck & Co., Inc.
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| Department |
Computational & Systems Biology
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| Street address |
33 Avenue Louis Pasteur
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02176 |
| Country |
USA |
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| Platforms (2) |
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| Samples (71)
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| GSM155601 |
HeLa, mock vs. HeLa, Luc, 200 nM, 24 hr |
| GSM155602 |
HeLa, mock vs. HeLa, miR_16, 200 nM, 24 hr |
| GSM155603 |
HeLa, mock vs. HeLa, miR_16-2-OME, 200 nM, 24 hr |
| GSM155604 |
HeLa, mock vs. HeLa, miR_106b, 200 nM, 24 hr |
| GSM155605 |
HeLa, mock vs. HeLa, miR_106b-2-OME, 200 nM, 24 hr |
| GSM156513 |
HCT116 Dicer -/- #2, mock, 6 hr vs. HCT116 Dicer -/- #2, Luc, 100 nM, 6 hr |
| GSM156514 |
HCT116 Dicer -/- #2, mock, 6 hr vs. HCT116 Dicer -/- #2, miR_16, 100 nM, 6 hr |
| GSM156515 |
HCT116 Dicer -/- #2, mock, 6 hr vs. HCT116 Dicer -/- #2, miR_15a, 100 nM, 6 hr |
| GSM156516 |
HCT116 Dicer -/- #2, mock, 6 hr vs. HCT116 Dicer -/- #2, miR_106b, 100 nM, 6 hr |
| GSM156517 |
HCT116 Dicer -/- #2, mock, 10 hr vs. HCT116 Dicer -/- #2, Luc, 100 nM, 10 hr |
| GSM156518 |
HCT116 Dicer -/- #2, mock, 10 hr vs. HCT116 Dicer -/- #2, miR_16, 100 nM, 10 hr |
| GSM156519 |
HCT116 Dicer -/- #2, mock, 10 hr vs. HCT116 Dicer -/- #2, miR_15a, 100 nM, 10 hr |
| GSM156520 |
HCT116 Dicer -/- #2, mock, 10 hr vs. HCT116 Dicer -/- #2, miR_106b, 100 nM, 10 hr |
| GSM156521 |
HCT116 Dicer -/- #2, mock, 24 hr vs. HCT116 Dicer -/- #2, Luc, 100 nM, 24 hr |
| GSM156522 |
HCT116 Dicer -/- #2, mock, 24 hr vs. HCT116 Dicer -/- #2, miR_16, 100 nM, 24 hr |
| GSM156523 |
HCT116 Dicer -/- #2, mock, 24 hr vs. HCT116 Dicer -/- #2, miR_15a, 100 nM, 24 hr |
| GSM156524 |
HCT116 Dicer -/- #2, mock, 24 hr vs. HCT116 Dicer -/- #2, miR_106b, 100 nM, 24 hr |
| GSM156525 |
HCT116 Dicer -/- #2, mock, 14 hr vs. HCT116 Dicer -/- #2, Luc, 100 nM, 14 hr |
| GSM156526 |
HCT116 Dicer -/- #2, mock, 14 hr vs. HCT116 Dicer -/- #2, miR_16, 100 nM, 14 hr |
| GSM156527 |
HCT116 Dicer -/- #2, mock, 14 hr vs. HCT116 Dicer -/- #2, miR_15a, 100 nM, 14 hr |
| GSM156528 |
HCT116, mock, 24 hr vs. HCT116, siRNA_200a, 100 nM, 24 hr |
| GSM156529 |
HCT116, mock, 24 hr vs. HCT116, siRNA_200b, 100 nM, 24 hr |
| GSM156530 |
HCT116, mock, 24 hr vs. HCT116, siRNA_141, 100 nM, 24 hr |
| GSM156531 |
HCT116/Dicer -/- #2, mock, 24 hr vs. HCT116/Dicer -/- #2, Luc, 100 nM, 24 hr |
| GSM156532 |
HCT116/Dicer -/- #2, mock, 24 hr vs. HCT116/Dicer -/- #2, siRNA_200a, 100 nM, 24 hr |
| GSM156533 |
HCT116/Dicer -/- #2, mock, 24 hr vs. HCT116/Dicer -/- #2, siRNA_200b, 100 nM, 24 hr |
| GSM156534 |
HCT116/Dicer -/- #2, mock, 24 hr vs. HCT116/Dicer -/- #2, siRNA_141, 100 nM, 24 hr |
| GSM156535 |
HCT116, mock, 16 hr vs. HCT116, KIF11_212 siRNA, 100 nM, 16 hr |
| GSM156536 |
HCT116 Dicer -/- #2, mock, 16 hr vs. HCT116 Dicer -/- #2, KIF11_212 siRNA, 100 nM, 16 hr |
| GSM156537 |
HCT116, mock, 22 hr vs. HCT116, Luc, 100nM, 22 hr |
| GSM156538 |
HCT116, mock, 22 hr vs. HCT116, hsa_miR_106b, 100nM, 22 hr |
| GSM156539 |
HCT116 Dicer -/- #2, mock, 22 hr vs. HCT116 Dicer -/- #2, hsa_miR_106b, 22 hr |
| GSM156540 |
HCT116 Dicer -/- #2, mock, 22 hr vs. HCT116 Dicer -/- #2, hsa_miR_103, 22 hr |
| GSM156541 |
DLD-1 Dicer -/- #6, mock, 25 nM, 24 hr vs. DLD-1 Dicer -/- #6, hsa_miR_200a, 25 nM, 24 hr |
| GSM156542 |
DLD-1 Dicer -/- #6, mock, 25 nM, 24 hr vs. DLD-1 Dicer -/- #6, hsa_miR_141, 25 nM, 24 hr |
| GSM156543 |
DLD-1 Dicer -/- #6, mock, 25 nM, 24 hr vs. DLD-1 Dicer -/- #6, hsa_miR_106b, 25 nM, 24 hr |
| GSM156544 |
DLD-1 Dicer -/- #6, mock, 25 nM, 24 hr vs. DLD-1 Dicer -/- #6, hsa_miR_103, 25 nM, 24 hr |
| GSM156545 |
HCT116 Dicer -/- #2, mock, 24 hr vs. HCT116 Dicer -/- #2, hsa-miR-15a, 100 nM, 24 hr |
| GSM156546 |
HCT116 Dicer -/- #2, mock, 24 hr vs. HCT116 Dicer -/- #2, hsa-miR-16, 100 nM, 24 hr |
| GSM156547 |
HCT116 Dicer -/- #2, mock, 24 hr vs. HCT116 Dicer -/- #2, hsa-miR-192, 100 nM, 24 hr |
| GSM156548 |
HCT116 Dicer -/- #2, mock, 24 hr vs. HCT116 Dicer -/- #2, hsa-miR-215, 100 nM, 24 hr |
| GSM156549 |
DLD-1 Dicer -/- #6, mock, 25 nM, 24 hr vs. DLD-1 Dicer -/- #6, hsa-miR-15a, 25 nM, 24 hr |
| GSM156550 |
DLD-1 Dicer -/- #6, mock, 25 nM, 24 hr vs. DLD-1 Dicer -/- #6, hsa-miR-16, 25 nM, 24 hr |
| GSM156551 |
DLD-1 Dicer -/- #6, mock, 25 nM, 24 hr vs. DLD-1 Dicer -/- #6, hsa-miR-192, 25 nM, 24 hr |
| GSM156552 |
DLD-1 Dicer -/- #6, mock, 25 nM, 24 hr vs. DLD-1 Dicer -/- #6, hsa-miR-215, 25 nM, 24 hr |
| GSM156553 |
HCT116 Dicer -/- #2, mock, 24 hr vs. HCT116 Dicer -/- #2, hsa-miR-17-5p, 100 nM, 24 hr |
| GSM156554 |
HCT116 Dicer -/- #2, mock, 24 hr vs. HCT116 Dicer -/- #2, hsa-miR-20, 100 nM, 24 hr |
| GSM156555 |
DLD-1 Dicer -/- #6, mock, 24 hr vs. DLD-1 Dicer -/- #6, hsa-miR-17-5p, 25 nM, 24 hr |
| GSM156556 |
DLD-1 Dicer -/- #6, mock, 24 hr vs. DLD-1 Dicer -/- #6, hsa-miR-20, 25 nM, 24 hr |
| GSM156557 |
HCT116 Dicer -/- #2, mock, 24 hr vs. HCT116 Dicer -/- #2, hsa-let-7c, 100 nM, 24 hr |
| GSM156558 |
DLD-1 Dicer -/- #6, mock, 24 hr vs. DLD-1 Dicer -/- #6, hsa-let-7c, 25 nM, 24 hr |
| GSM156559 |
HCT116 Dicer -/- #2, mock vs. HCT116 Dicer -/- #2, miR_195, 100 nM, 24 hr |
| GSM156563 |
HCT116 Dicer -/- #2, mock, 24 hr vs. HCT116 Dicer -/- #2, miR_16_mm2,3, 100 nM, 24 hr |
| GSM156564 |
HCT116 Dicer -/- #2, mock, 24 hr vs. HCT116 Dicer -/- #2, miR_16_mm4,5, 100 nM, 24 hr |
| GSM156565 |
HCT116 Dicer -/- #2, mock, 24 hr vs. HCT116 Dicer -/- #2, miR_16_mm18,19, 100 nM, 24 hr |
| GSM156566 |
HCT116 Dicer -/- #2, mock, 24 hr vs. HCT116 Dicer -/- #2, miR_16_mm19,20, 100 nM, 24 hr |
| GSM156567 |
HCT116 Dicer -/- #2, mock vs. HCT116 Dicer -/- #2, Luc, 100 nM, 10 hr, b |
| GSM156568 |
HCT116 Dicer -/- #2, mock vs. HCT116 Dicer -/- #2, miR_106b, 100 nM, 10 hr, b |
| GSM156569 |
HCT116 Dicer -/- #2, mock vs. HCT116 Dicer -/- #2, miR_15a, 100 nM, 10 hr, b |
| GSM156570 |
HCT116 Dicer -/- #2, mock vs. HCT116 Dicer -/- #2, miR_15b, 100 nM, 10 hr |
| GSM156571 |
HCT116 Dicer -/- #2, mock vs. HCT116 Dicer -/- #2, miR_16, 100 nM, 10 hr, b |
| GSM156572 |
HCT116 Dicer -/- #2, mock vs. HCT116 Dicer -/- #2, miR_103, 100 nM, 10 hr |
| GSM156573 |
HCT116 Dicer -/- #2, mock vs. HCT116 Dicer -/- #2, miR_107, 100 nM, 10 hr |
| GSM156574 |
HCT116 Dicer -/- #2, mock vs. HCT116 Dicer -/- #2, miR_195, 100 nM, 10 hr |
| GSM156575 |
HCT116 Dicer -/- #2, mock vs. HCT116 Dicer -/- #2, Luc, 100 nM, 24 hr, b |
| GSM156576 |
HCT116 Dicer -/- #2, mock vs. HCT116 Dicer -/- #2, miR_106b, 100 nM, 24 hr, b |
| GSM156577 |
HCT116 Dicer -/- #2, mock vs. HCT116 Dicer -/- #2, miR_15a, 100 nM, 24 hr, b |
| GSM156578 |
HCT116 Dicer -/- #2, mock vs. HCT116 Dicer -/- #2, miR_15b, 100 nM, 24 hr |
| GSM156579 |
HCT116 Dicer -/- #2, mock vs. HCT116 Dicer -/- #2, miR_16, 100 nM, 24 hr, b |
| GSM156580 |
HCT116 Dicer -/- #2, mock vs. HCT116 Dicer -/- #2, miR_103, 100 nM, 24 hr |
| GSM156581 |
HCT116 Dicer -/- #2, mock vs. HCT116 Dicer -/- #2, miR_107, 100 nM, 24 hr |
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| Relations |
| BioProject |
PRJNA99129 |
| Supplementary data files not provided |
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