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| Status |
Public on Jun 11, 2015 |
| Title |
Transcriptional profile of XBP1-deficient ovarian cancer-associated dendritic cells (DCs) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
ID8-based ovarian tumors were developed for 3 weeks in wild type (WT, N=3) or conditional knockout mice selectively deleting XBP1 in CD11c positive cells (KO, N=3). Tumor-associated DCs were independently sorted via FACS and used for transcriptional profiling.
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| Overall design |
Total RNA from sorted tumor-associated DCs (N=3/genotype) was independently isolated using the miRVANA kit (Life Technologies) and further purified and concentrated using minElute columns (Qiagen). RNA integrity was confirmed using an Agilent Bioanalyzer 2100.
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| |
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| Contributor(s) |
Cubillos-Ruiz JR, Glimcher LH |
| Citation(s) |
26073941 |
| Submission date |
May 01, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Juan R Cubillos-Ruiz |
| E-mail(s) |
jrcubillos@gmail.com
|
| Organization name |
Weill Cornell Medicine
|
| Department |
Medicine
|
| Street address |
1300 York Ave, E-907
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA282806 |
| SRA |
SRP057879 |
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