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Series GSE6850 Query DataSets for GSE6850
Status Public on Jun 15, 2007
Title A dominant negative form of cJun affects genes that have opposing effects on lipid homeostasis in mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary cJun is a transcription factor activated by phosphorylation by SAPK/JNK MAP kinase pathway that has been linked to atherosclerosis. Adenovirus mediated gene transfer of a dominant negative form of cJun in C57BL/6 mice increased greatly the apolipoprotein E (ApoE) mRNA and plasma apoE levels and induced dyslipidmia, characterized by increased plasma cholesterol, triglyceride and VLDL levels and accumulation of discoidal HDL particles. Unexpectedly, infection of ApoE-/- mice with adenovirus expressing dn-cJun reduced by 50% plasma cholesterol, suggesting that the dn-cJun affected other genes that control plamsa cholesterol. To determine the molecular pathways implicated in this process we performed whole genome expression profiling using total RNA from the liver of infected ApoE-/- mice.
Keywords: disease
 
Overall design We extracted total RNA from 5 ApoE-/- mice infected with an adenovirus expressing dominant negative cJun and 5 ApoE-/- mice infected with a control adenovirus expressing GFP. Each RNA sample was hybridized to a different Affymetrix 430 2.0 array.
 
Contributor(s) Drosatos K, Sanoudou D, Kypreos K, Kardassis D, Zannis V
Citation(s) 17456467
Submission date Jan 24, 2007
Last update date Feb 11, 2019
Contact name Despina Sanoudou
E-mail(s) dsanoudo@enders.tch.harvard.edu
Phone +30-210-6597453
Fax +30-210-6597545
Organization name Foundation for Biomedical Research, Academy of Athens
Department Molecular Biology
Lab Sanoudou Lab
Street address Soranou Efesiou 4
City Athens
ZIP/Postal code 115 27
Country Greece
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (10)
GSM157850 Control VZ11
GSM157857 Control VZ12
GSM157858 Control VZ13
Relations
BioProject PRJNA99091

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6850_RAW.tar 58.2 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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