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| Status |
Public on Mar 14, 2018 |
| Title |
Expression data from HEK 293T cells overexpressing either myc-FUS or myc-R495X (exon) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Individuals with the ALS-linked (amyotrophic lateral sclerosis) truncation mutation (R495X) in FUS (fused in sarcoma) are known to have a more aggressive form of the disease than those with point mutations. The underlying cause for this difference is unclear. We report that FUS is a component of miRISC (miRNA-induced silencing complex) and that overexpression of its truncation mutant R495X negatively impacts miRNA mediated RNA silencing.
In this study, we compared the impact overexpression of wild-type myc-tagged FUS versus truncation mutant R495X has on global gene expression.
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| Overall design |
Pairwise comparisons were made based on three biological replicates from two groups: 293Ts expressing myc-tagged R495x versus those expressing wild-type myc-tagged FUS.
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| Contributor(s) |
Mullane PM, Wang J |
| Citation(s) |
29499134 |
| Submission date |
May 04, 2015 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Jiou Wang |
| Organization name |
Johns Hopkins University
|
| Street address |
615 N. Wolfe St.
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE68504 |
Expression data from HEK 293T cells overexpressing either myc-FUS or myc-R495X |
|
| Relations |
| BioProject |
PRJNA282942 |
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