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Series GSE68633 Query DataSets for GSE68633
Status Public on Feb 11, 2017
Title Gene expression profiling of the human HepaRG hepatocellular carcinoma cell line under WNT3A stimulation.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The aim of the study was to characterize at a molecular level (changes in mRNA level) the effects of WNT3A on the human HepaRG hepatocellular carcinoma cell line.
This was adressed by culturing HepaRG cells in presence or absence of Wnt3a.
 
Overall design HepaRG cells were treated with 200 ng/ml Wnt3a for 72hrs. To dissect the gene networks involved, experiments were also performed to silence β-catenin expression with a specific siRNA and to inhibit Wnt3a in the extracellular space with FZD8_CRD, a soluble Wnt-binding domain with SFRP-like functions. HepaRG cells were plated three days before siRNA transfection and treated with 200 ng/ml Wnt3a alone and/or 300 ng/ml FZD8_CRD from day one through day three after transfection. Independent culture experiments were performed in triplicate.
 
Contributor(s) Coulouarn C
Citation(s) 27191501
Submission date May 07, 2015
Last update date Jan 09, 2018
Contact name Cedric Coulouarn
E-mail(s) cedric.coulouarn@inserm.fr
Organization name INSERM
Street address Hôpital Pontchaillou
City Rennes
ZIP/Postal code 35033
Country France
 
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (24)
GSM1677865 HepaRG_untransfected_untreated_replicate1
GSM1677866 HepaRG_transfected-CTRLsiRNA_untreated_replicate1
GSM1677867 HepaRG_transfected-CTNNB1siRNA_untreated_replicate1
Relations
BioProject PRJNA283271

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68633_RAW.tar 72.9 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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