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| Status |
Public on May 01, 2018 |
| Title |
Transcriptome of mouse epithelial cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We have compared the transcriptional profiles from three epithelial cell types to find common and differentially expressed genes. Epithelial cells from small intestine and kidney from C57BL/6 mice were sorted and their transcriptional profiles compared. Cells were gated as viable, Epcam+ CD45- for kidney, and viable, Epcam+ CD45- MHCIIhigh for small intestine MHCIIhi and viable, Epcam int CD45- MHCII low for small intestine MHCIIlo.
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| Overall design |
Three independent replicates were analysed per group. Kidney epithelial cells: kidneys were minced, digested with collagenase, dispase and DNAse. Supernatants were stained with Epcam and CD45 for FACSsort; Fluorogold was used to exclude dead cells. Small intestinal epithelial cells: small intestines were cut open, contents removed and minced. Fragments were washed and incubated with EDTA, and supernatants were stained with Epcam, CD45 and MHCII; Fluorogold was used to exclude dead cells. For small intestine, cells from 3 mice were pooled per replicate. For Kidney, cells from 4 mice were pooled per replicate.
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| Contributor(s) |
Izcue A, Rauf P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 08, 2015 |
| Last update date |
May 10, 2018 |
| Contact name |
Ana Izcue |
| Organization name |
Max Planck Institut of Immunobiology and Epigenetics
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| Street address |
Am Stübeweg 51
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| City |
Freiburg |
| ZIP/Postal code |
79108 |
| Country |
Germany |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA283383 |
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