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| Status |
Public on May 09, 2015 |
| Title |
Gene expression profiling in the liver of 3-month old mice with a mutant Wrn protein treated with vitamin C compared to the liver of 3-month old untreated mice with a mutant Wrn protein. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Werner syndrome (WS) is a rare disorder characterized by the premature onset of a number of age-related diseases. The gene responsible for WS is believed to be involved in different aspects of transcription, replication, and/or DNA repair. We generated a mouse model with a deletion in the helicase domain of the murine WRN homologue that recapitulates most of the WS phenotypes including an abnormal hyaluronic acid excretion, higher reactive oxygen species (ROS) levels, increased genomic instability and cancer incidence resulting in a 10-15% decreased life span expectancy. In addition, WS patients and Wrn mutant mice show hallmarks of a metabolic syndrome including premature visceral obesity, hypertriglyceridemia, insulin-resistant diabetes type 2 and associated cardiovascular diseases. In this study, we compared the expression profile of liver tissues from 3 months old Wrn mutant mice treated with 0.4% vitamin C to untreated 3 months old Wrn mutant mice.
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| Overall design |
Microarray analyses were performed on the liver tissues of 3 months old mice. Four independent biological replicates of this experiment (untreated Wrn mutant mice vs vitamin C treated Wrn mutant mice) were carried out on four replicates of each genotype.
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| Contributor(s) |
Paquet ER, Lebel M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 08, 2015 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Michel Lebel |
| E-mail(s) |
michel.lebel@crchudequebec.ulaval.ca
|
| Phone |
(418) 525-4444
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| Organization name |
Centre de Recherche du CHU de Québec
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| Department |
Centre Hospitalier de l’Université Laval (CHUL)
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| Lab |
Michel Lebel
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| Street address |
2705 Laurier Blvd, Local T-2-04
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| City |
Quebec |
| State/province |
Qc |
| ZIP/Postal code |
G1V 4G2 |
| Country |
Canada |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (4)
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| GSM1679051 |
liver Wrn mutant vitamin C #1 / liver Wrn mutant #1 |
| GSM1679052 |
liver Wrn mutant vitamin C #2 / liver Wrn mutant #2 |
| GSM1679053 |
liver Wrn mutant vitamin C #3 / liver Wrn mutant #3 |
| GSM1679054 |
liver Wrn mutant vitamin C #4 / liver Wrn mutant #4 |
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| Relations |
| BioProject |
PRJNA283398 |
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