|
| Status |
Public on Jan 26, 2007 |
| Title |
Development of Regulatory T cell Precursors in the Absence of a Functional Foxp3 Protein |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To analyze gene expression in in regulatory T cell precursors that develop in the absence of a functional Foxp3 protein as compared to that of normal regulatory T cells
Keywords: Cell type comparison
|
| |
|
| Overall design |
Murine wild-type and mutant (delta) Foxp3 alleles were tagged with EGFP reporters. CD4 positive T cells expressing the respective tagged Foxp3 allele (Foxp3/EGFP and delta Foxp3/EGFP) were isolated from hemizygous male mice by cell sorting using FACS. CD4 positive cells that did not express the EGFP tag were a isolated. Total RNA was prepared and used in the array studies.
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| |
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| Contributor(s) |
Lin W, Haribhai D, Reland LM, Truong N, Carlson MR, Williams CB, Chatila TA |
| Citation(s) |
17273171 |
| Submission date |
Jan 25, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Marc Richard John Carlson |
| Organization name |
UCLA
|
| Department |
Department of Human Genetics
|
| Lab |
Stan Nelson Laboratory
|
| Street address |
695 Charles E. Young Dr. S.
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (8)
|
| GSM158519 |
76090_CD4+, FoxP3(EGFP-) mouse cells |
| GSM158520 |
76091_CD4+, FoxP3(EGFP+) mouse cells |
| GSM158521 |
76092_CD4+, (delta)FoxP3(EGFP-) mouse cells |
| GSM158522 |
76093_CD4+, (delta)FoxP3(EGFP+) mouse cells |
| GSM158523 |
82428_CD4+, FoxP3(EGFP+) mouse cells.txt |
| GSM158525 |
82430_CD4+, (delta)FoxP3(EGFP+) mouse cells |
| GSM158527 |
85382_CD4+, FoxP3(EGFP+) mouse cells |
| GSM158528 |
85384_CD4+, (delta)FoxP3(EGFP+) mouse cells |
|
| Relations |
| BioProject |
PRJNA99127 |
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